Rabbit AXL/UFO Polyclonal Antibody | anti-AXL/UFO antibody

Rabbit anti AXL/UFO (Phospho) Polyclonal Antibody

Cat. Num. AAA460556

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by site-modified Epitope Affinity Purification.
• Synonyms: AXL/UFO; Polyclonal Antibody; Rabbit anti AXL/UFO (Phospho) Polyclonal Antibody; AXL receptor tyrosine kinase; UFO; anti-AXL/UFO antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes AXL (pY779) with a phosphorylated site at Tyrosine 779. It does not crossreact with non-phosphospecific peptide.
• Purity/Purification: Purified by site-modified Epitope Affinity Purification.
• Form/Format: Supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.

• Applicable Applications for anti-AXL/UFO antibody: ELISA (EIA), Flow Cytometry (FC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Immunohistochemistry: 2-5 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml; Optimal dilutions should be determined by researchers for the specific applications
• Antigen Preperation: A synthetic peptide surrounding to the epitope -GL YAL- with a phosphorylation site at Tyr779 of human AXL protein . This sequence is identical among human, mouse, rat.
• Positive Control: EGF stimulated HUVEC cell lysate
• Cellular Location: Cytoplasmic
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Western Blot (WB)

(Western Blot: The EGF stimulated HUVEC cell Iysates were resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with Rabbit anti AXL(Phosphospecific) (Cat# MBS460556) antibody (Lane 1 &2 ) at 1 :500.)

Related Product Information for anti-AXL/UFO antibody

Background: AXL is a member of the Tyr03-Axl-Mer (TAM) receptor tyrosine kinase subfamily. It possesses an extracellu lar domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6), and which is thus regulating many physiological processes including cell survival, cell proliferation , migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation ofAXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of Pl3-kinase subunits PIK3R1 , PIK3R2 and PIK3R3; but also GRB2, PLCG1 , LCK and PTPN11 . Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration , gonadotropin-releasing hormone neuron survival and migration , platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor. AXLlUFO may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types.