Rabbit MAPKK5 (MEK5) (pS311/pT315) Antibody | anti-MAPKK5 (MEK5) (pS311/pT315) antibody

Rabbit anti MEK5 (pSer311/ pThr315, Phospho-specific)

Cat. Num. AAA462077

• Gene Names: MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454
• Reactivity: Human, Rat, Mouse, Chicken, Canine
• Applications: ELISA, Western Blot
• Purity: The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
• Synonyms: MAPKK5 (MEK5) (pS311/pT315); Rabbit anti MEK5 (pSer311/ pThr315; Phospho-specific); Rabbit anti-MEK5 (pSer311pThr315); MAPKK5; MEK5; MKK5; MAPK/ERK Kinase 5; SKK5.; anti-MAPKK5 (MEK5) (pS311/pT315) antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Chicken, Canine
• Specificity: This antibody recognizes ~49 kDa of human MEK5 protein at the phosphorylation site of Ser 311/Thr315. It does not cross react to non-phospho MEK5. It also reacts with mouse and rat, chicken. The other species are not tested.
• Purity/Purification: The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-MAPKK5 (MEK5) (pS311/pT315) antibody: ELISA, Western Blot
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 2-5 ug/ml
• Antigen Preparation: A synthetic peptide surrounding the epitope -SIAKT- with double phosphorylation sites Ser311 and Thr315. This sequence is identical among human, rat, mouse, chicken, and dog.
• Positive Control: EGF-stimulated A431
• Cellular Location: Cytoplasmic
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-MAPKK5 (MEK5) (pS311/pT315) antibody

The MEK5 (also known as MKK5, MAPKK5, SKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. It catalyzes tyrosine phosphorylation of ERK5 whereas MEK5 itself is regulated by MEKK3. An important link between MEK5 and metastatic prostrate cancer has been demonstrated. Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation.

References

Tetsuro Shishido, et al. Effects of MEK5/ERK5 Association on Small Ubiquitin-Related Modification of ERK5: Implications for Diabetic Ventricular Dysfunction After Myocardial Infarction. Circulation Research. 2008; 102:1416.

NCBI and Uniprot Product Information

• NCBI GI #: 21729895
• NCBI GeneID: 5607
• NCBI Accession #: NP_660143.1
• NCBI GenBank Nucleotide #: NM_145160.1
• UniProt Accession #: Q13163; Q92961; Q92962
• Molecular Weight: 49 kDa
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 5 isoform A
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 5
• NCBI Official Symbol: MAP2K5
• NCBI Official Synonym Symbols: MEK5; MAPKK5; PRKMK5; HsT17454
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 5; MEK 5; MAPKK 5; MAPK/ERK kinase 5; OTTHUMP00000164363; MAP kinase kinase 5; MAP kinase kinase MEK5b
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 5
• UniProt Gene Name: MAP2K5
• UniProt Synonym Gene Names: MEK5; MKK5; PRKMK5
• UniProt Entry Name: MP2K5_HUMAN

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq]

Uniprot Description

Function: Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis. Ref.1 Ref.2

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Subunit structure: Interacts with PARD6A, MAP3K3 and MAPK7. Forms a complex with SQSTM1 and PRKCZ or PRKCI

By similarity. Interacts with Yersinia yopJ. Ref.1 Ref.7

Tissue specificity: Expressed in many adult tissues. Abundant in heart and skeletal muscle.

Domain: Binds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the OPR domain. This domain also mediates interactions with SQSTM1 and PARD6A

Post-translational modification: Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases

By similarity.Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 OPR domain.Contains 1 protein kinase domain.