Mouse G Protein Coupled Estrogen Receptor 1 (GPER) RTU ELISA Kit | GPER rtu elisa kit

Mouse G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit

Cat. Num. AAA459676

• Gene Names: Gper1; Gper; Ceprl; FEG-1; Gpr30; CMKRL2; GPCR-Br; 6330420K13Rik
• Reactivity: Mouse
• Synonyms: G Protein Coupled Estrogen Receptor 1 (GPER); Mouse G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit; GPR30; CMKRL2; DRY12; FEG-1; GPCR-Br; LERGU; LyGPR; Membrane estrogen receptor; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1; GPER rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 375

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPER rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPER. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GPER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GPER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GPER in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 254588034
• NCBI GeneID: 76854
• NCBI Accession #: NP_084047.2
• NCBI GenBank Nucleotide #: NM_029771.3
• UniProt Accession #: Q8BMP4; Q9D392; B2RRW0
• Molecular Weight: 42,479 Da
• NCBI Official Full Name: G-protein coupled estrogen receptor 1
• NCBI Official Synonym Full Names: G protein-coupled estrogen receptor 1
• NCBI Official Symbol: Gper1
• NCBI Official Synonym Symbols: Gper; Ceprl; FEG-1; Gpr30; CMKRL2; GPCR-Br; 6330420K13Rik
• NCBI Protein Information: G-protein coupled estrogen receptor 1
• UniProt Protein Name: G-protein coupled estrogen receptor 1
• UniProt Gene Name: Gper1
• UniProt Synonym Gene Names: Cmkrl2; Gper; Gpr30; mER

Uniprot Description

G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK. Mediates pleiotropic functions among others in the cardiovascular, endocrine, reproductive, immune and central nervous systems. Has a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a RAMP3-dependent manner. Regulates arterial blood pressure by stimulating vasodilation and reducing vascular smooth muscle and microvascular endothelial cell proliferation. Plays a role in blood glucose homeostasis contributing to the insulin secretion response by pancreatic beta cells. Triggers mitochondrial apoptosis during pachytene spermatocyte differentiation. Stimulates uterine epithelial cell proliferation. Enhances uterine contractility in response to oxytocin. Contributes to thymic atrophy by inducing apoptosis. Attenuates TNF-mediated endothelial expression of leukocyte adhesion molecules. Promotes neuritogenesis in developing hippocampal neurons. Plays a role in acute neuroprotection against NMDA-induced excitotoxic neuronal death. Increases firing activity and intracellular calcium oscillations in luteinizing hormone-releasing hormone (LHRH) neurons. Inhibits early osteoblast proliferation at growth plate during skeletal development. Inhibits mature adipocyte differentiation and lipid accumulation. Involved in the recruitment of beta-arrestin 2 ARRB2 at the plasma membrane in epithelial cells. Functions also as a receptor for aldosterone mediating rapid regulation of vascular contractibility through the PI3K/ERK signaling pathway. Involved in cancer progression regulation. Stimulates cancer-associated fibroblast (CAF) proliferation by a rapid genomic response through the EGFR/ERK transduction pathway. Associated with EGFR, may act as a transcription factor activating growth regulatory genes (c-fos, cyclin D1). Promotes integrin alpha-5/beta-1 and fibronectin (FN) matrix assembly in breast cancer cells.