Rabbit anti-Human, Mouse USP15 Polyclonal Antibody | anti-USP15 antibody

USP15, NT (USP15, KIAA0529, Ubiquitin carboxyl-terminal hydrolase 15, Deubiquitinating enzyme 15, Ubiquitin thioesterase 15, Ubiquitin-specific-processing protease 15, Unph-2, Unph4) (APC)

Cat. Num. AAA6361851

• Gene Names: USP15; UNPH4; UNPH-2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: USP15; Polyclonal Antibody; NT (USP15; KIAA0529; Ubiquitin carboxyl-terminal hydrolase 15; Deubiquitinating enzyme 15; Ubiquitin thioesterase 15; Ubiquitin-specific-processing protease 15; Unph-2; Unph4) (APC); Unph4; anti-USP15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-USP15 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: USP15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the N-terminal region of human USP15.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of USP15 (arrow) using rabbit polyclonal USP15 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the USP15 gene.)

Western Blot (WB)

(USP15 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the USP15 antibody detected the USP15 protein (arrow).)

Related Product Information for anti-USP15 antibody

Ubiquitin (MIM 191339), a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). Also see USP1 (MIM 603478).

Product Categories/Family for anti-USP15 antibody

Antibodies; Ubiquitin

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Vos, R.M., et al. J. Virol. 83(17):8885-8892(2009). Huang, X., et al. J. Mol. Biol. 391(4):691-702(2009). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 14149627
• NCBI GeneID: 9958
• NCBI Accession #: NP_006304.1
• NCBI GenBank Nucleotide #: NM_006313.2
• UniProt Accession #: Q9Y4E8; Q08AL5; Q9H8G9; Q9HCA6; Q9UNP0; Q9Y5B5
• Molecular Weight: 27,094 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 15 isoform 2
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 15
• NCBI Official Symbol: USP15
• NCBI Official Synonym Symbols: UNPH4; UNPH-2
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 15; ubiquitin thioesterase 15; deubiquitinating enzyme 15; ubiquitin thiolesterase 15; ubiquitin-specific-processing protease 15
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 15
• UniProt Gene Name: USP15
• UniProt Synonym Gene Names: KIAA0529
• UniProt Entry Name: UBP15_HUMAN

NCBI Description

This gene encodes a member of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. USP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The encoded protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2. [provided by RefSeq, Nov 2011]

Uniprot Description

USP15: Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Ubiquitin-specific protease; EC 3.4.19.12; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; SMAD binding; transforming growth factor beta receptor binding

Biological Process: BMP signaling pathway; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; transforming growth factor beta receptor signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway