Rabbit MSH6 Antibody | anti-MSH6 antibody

MSH6 Antibody

Cat. Num. AAA8504172

• Gene Names: MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: MSH6; MSH6 Antibody; GTBP; HNPCC5; HSAP; anti-MSH6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 1360

• Applicable Applications for anti-MSH6 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human MSH6
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various celllines, using MSH6 antibody.)

Related Product Information for anti-MSH6 antibody

The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between nonidentical DNA sequences, and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-alpha dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-alpha is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins, and the Rad50-Mre11-NBS1 complex (2). Mutations in MSH6 and other MMR proteins have been found in a large proportion of hereditary nonpolyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations in MSH6 have been shown to occur in glioblastoma in response to temozolomide therapy and to promote temozolomide resistance (4).

Product Categories/Family for anti-MSH6 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. O'Brien, V. and Brown, R. (2006) Carcinogenesis 27, 682-92. 2. Wang, Y. et al. (2000) Genes Dev 14, 927-39. 3. Plotz, G. et al. (2006) J Mol Histol 37, 271-83. 4. Yip, S. et al. (2009) Clin Cancer Res 15, 4622-9.

NCBI and Uniprot Product Information

• NCBI GI #: 4504191
• NCBI GeneID: 2956
• NCBI Accession #: NP_000170.1
• NCBI GenBank Nucleotide #: NM_000179.2
• UniProt Accession #: P52701; O43706; O43917; Q8TCX4; Q9BTB5; B4DF41; B4E3I4; F5H2F9
• Molecular Weight: 153kDa
• NCBI Official Full Name: DNA mismatch repair protein Msh6 isoform 1
• NCBI Official Synonym Full Names: mutS homolog 6
• NCBI Official Symbol: MSH6
• NCBI Official Synonym Symbols: GTBP; HSAP; p160; GTMBP; HNPCC5
• NCBI Protein Information: DNA mismatch repair protein Msh6
• UniProt Protein Name: DNA mismatch repair protein Msh6
• Protein Family: DNA mismatch repair protein
• UniProt Gene Name: MSH6
• UniProt Synonym Gene Names: p160

NCBI Description

This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]

Uniprot Description

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.