Typical Testing Data/Standard Curve (for reference only)
Human G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit | GPER elisa kit
Human G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GPER. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GPER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GPER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GPER in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum and a member of the G-protein coupled receptor 1 family. This receptor binds estrogen and activates multiple downstream signaling pathways, leading to stimulation of adenylate cyclase and an increase in cyclic AMP levels, while also promoting intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. This receptor has been shown to play a role in diverse biological processes, including bone and nervous system development, metabolism, cognition, male fertility and uterine function. [provided by RefSeq, Aug 2017]
Uniprot Description
GPER1: Receptor for estrogen. Belongs to the G-protein coupled receptor 1 family.
Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Receptor, GPCR
Chromosomal Location of Human Ortholog: 7p22.3
Cellular Component: axon; axon terminus; cell junction; cytoplasm; cytoplasmic vesicle membrane; dendrite; dendritic shaft; early endosome; endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus; Golgi membrane; integral component of plasma membrane; intracellular; keratin filament; mitochondrial membrane; nuclear envelope; nucleus; perinuclear region of cytoplasm; plasma membrane; postsynaptic density; presynaptic active zone; presynaptic membrane; recycling endosome; trans-Golgi network
Molecular Function: chromatin binding; estrogen receptor activity; G-protein coupled receptor activity; mineralocorticoid receptor activity; protein binding; steroid binding
Biological Process: apoptotic chromosome condensation; cell cycle; elevation of cytosolic calcium ion concentration; G-protein coupled receptor signaling pathway; generation of action potential; inflammatory response; innate immune response; mineralocorticoid receptor signaling pathway; negative regulation of cell proliferation; negative regulation of DNA metabolic process; negative regulation of fat cell differentiation; negative regulation of inflammatory response; negative regulation of leukocyte activation; negative regulation of lipid biosynthetic process; negative regulation of protein kinase B signaling cascade; nuclear fragmentation involved in apoptotic nuclear change; positive regulation of apoptosis; positive regulation of cAMP biosynthetic process; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of inositol trisphosphate biosynthetic process; positive regulation of insulin secretion; positive regulation of MAPK cascade; positive regulation of neurogenesis; positive regulation of neurotransmitter secretion; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; regulation of cytosolic calcium ion concentration; steroid hormone mediated signaling; steroid hormone receptor signaling pathway
Research Articles on GPER
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Product Notes
The Human GPER gper1 (Catalog #AAA2706008) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2706008 ELISA Kit recognizes Human GPER. It is sometimes possible for the material contained within the vial of "G Protein Coupled Estrogen Receptor 1 (GPER), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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