Mouse G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit | GPER elisa kit

Mouse G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit

Cat. Num. AAA2020328

• Gene Names: GPER1; mER; CEPR; GPER; DRY12; FEG-1; GPR30; LERGU; LyGPR; CMKRL2; LERGU2; GPCR-Br
• Reactivity: Mouse
• Synonyms: G Protein Coupled Estrogen Receptor 1 (GPER); Mouse G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit; GPR30; CMKRL2; DRY12; FEG-1; GPCR-Br; LERGU; LyGPR; Membrane estrogen receptor; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1; GPER elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of G Protein Coupled Estrogen Receptor 1 (GPER).; No significant cross-reactivity or interference between G Protein Coupled Estrogen Receptor 1 (GPER) and analogues was observed.
• Sequence Length: 375

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level G Protein Coupled Estrogen Receptor 1 (GPER) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level G Protein Coupled Estrogen Receptor 1 (GPER) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPER elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Estrogen Receptor 1 (GPER). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Estrogen Receptor 1 (GPER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Estrogen Receptor 1 (GPER), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Coupled Estrogen Receptor 1 (GPER) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GPER elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 148228791
• NCBI GeneID: 2852
• NCBI Accession #: NP_001091671.1
• NCBI GenBank Nucleotide #: NM_001098201.1
• UniProt Accession #: Q99527; O00143; O43494; Q13631; Q6FHL1; Q96F42; Q99981; A8K6C5; B5BUJ1
• Molecular Weight: 42,248 Da
• NCBI Official Full Name: G-protein coupled estrogen receptor 1
• NCBI Official Synonym Full Names: G protein-coupled estrogen receptor 1
• NCBI Official Symbol: GPER1
• NCBI Official Synonym Symbols: mER; CEPR; GPER; DRY12; FEG-1; GPR30; LERGU; LyGPR; CMKRL2; LERGU2; GPCR-Br
• NCBI Protein Information: G-protein coupled estrogen receptor 1
• UniProt Protein Name: G-protein coupled estrogen receptor 1
• UniProt Gene Name: GPER1
• UniProt Synonym Gene Names: CEPR; CMKRL2; DRY12; GPER; GPR30; FEG-1; LYGPR; mER
• UniProt Entry Name: GPER1_HUMAN

NCBI Description

This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

GPER1: Receptor for estrogen. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; Motility/polarity/chemotaxis; GPCR, family 1

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: Golgi apparatus; integral to plasma membrane; dendrite; early endosome; nuclear envelope; trans-Golgi network; recycling endosome; perinuclear region of cytoplasm; keratin filament; cytoplasm; dendritic shaft; intracellular; endoplasmic reticulum membrane; cytoplasmic vesicle membrane; endoplasmic reticulum; postsynaptic density; Golgi membrane; presynaptic membrane; axon; mitochondrial membrane; plasma membrane; presynaptic active zone; nerve terminal; cell junction; nucleus

Molecular Function: G-protein coupled receptor activity; protein binding; estrogen receptor activity; chromatin binding; mineralocorticoid receptor activity; steroid binding

Biological Process: positive regulation of apoptosis; generation of action potential; nuclear fragmentation during apoptosis; positive regulation of caspase activity; positive regulation of vasodilation; negative regulation of DNA metabolic process; positive regulation of neurotransmitter secretion; negative regulation of leukocyte activation; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; positive regulation of MAPKKK cascade; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; inflammatory response; steroid hormone receptor signaling pathway; positive regulation of neurogenesis; cytosolic calcium ion homeostasis; negative regulation of lipid biosynthetic process; positive regulation of insulin secretion; positive regulation of inositol trisphosphate biosynthetic process; apoptotic chromosome condensation; negative regulation of fat cell differentiation; cell cycle; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; mineralocorticoid receptor signaling pathway; positive regulation of G-protein coupled receptor protein signaling pathway; negative regulation of inflammatory response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; positive regulation of protein amino acid phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cell migration