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Typical Testing Data/Standard Curve (for reference only)

Mouse Nestin (NES) RTU ELISA Kit | NES rtu elisa kit

Mouse Nestin (NES) ELISA Kit

Gene Names
Nes; RC2; Marc2; C78523; ESTM46; Ifaprc2; AA166324
Reactivity
Mouse
Synonyms
Nestin (NES); Mouse Nestin (NES) ELISA Kit; NES rtu elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Sequence Length
1864
Assay Type
Sandwich
Samples
Tissue Homogenates, Cell Lysates or Other Biological Fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.062ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NES rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NES. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NES in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
201,770 Da
NCBI Official Full Name
nestin
NCBI Official Synonym Full Names
nestin
NCBI Official Symbol
Nes
NCBI Official Synonym Symbols
RC2; Marc2; C78523; ESTM46; Ifaprc2; AA166324
NCBI Protein Information
nestin
UniProt Protein Name
Nestin
Protein Family
UniProt Gene Name
Nes

Uniprot Description

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division (). Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Research Articles on NES

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Product Notes

The Mouse NES nes (Catalog #AAA4501329) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA4501329 RTU ELISA Kit recognizes Mouse NES. It is sometimes possible for the material contained within the vial of "Nestin (NES), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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