Activin A ELISA Kit | INHBA elisa kit

Bovine Activin A PicoKine ELISA Kit

Cat. Num. AAA1751006

• Gene Names: INHBA; EDF; FRP
• Reactivity: Bovine; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: Activin A; Bovine Activin A PicoKine ELISA Kit; Inhibin; beta A (Activin A; activin AB alpha polypeptide); isoform CRA_a; cDNA FLJ75379; highly similar to Homo sapiens inhibin; beta A (activin A; mRNA; INHBA; hCG_17267; tcag7.474; INHBA elisa kit

• Reactivity: Bovine; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Bovine Bovine Activin A
• Sequence Length: 426

• Applicable Applications for INHBA elisa kit: ELISA (EIA)
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <12pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration weretested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration weretested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INHBA elisa kit

Principle of the Assay: Bovine Bovine Activin A ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Bovine Activin A has been precoated onto 96-well plates. Standards(Expression system for standard: CHO; Immunogen sequence: G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Bovine Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Bovine Bovine Activin A amount of sample captured in plate.

Background: Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.

Product Categories/Family for INHBA elisa kit

Signal Transduction; Growth Factors/Hormones; Cancer; Tumor Biomarkers

NCBI and Uniprot Product Information

• NCBI GI #: 4504699
• NCBI GeneID: 3624
• NCBI Accession #: NP_002183.1
• NCBI GenBank Nucleotide #: NM_002192.3
• UniProt Accession #: A4D1W7; P08476
• Molecular Weight: 47442 MW
• NCBI Official Full Name: inhibin beta A chain preproprotein
• NCBI Official Synonym Full Names: inhibin beta A subunit
• NCBI Official Symbol: INHBA
• NCBI Official Synonym Symbols: EDF; FRP
• NCBI Protein Information: inhibin beta A chain
• UniProt Protein Name: Inhibin, beta A (Activin A, activin AB alpha polypeptide)
• Protein Family: Inhibin
• UniProt Gene Name: INHBA

NCBI Description

This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate a subunit of the dimeric activin and inhibin protein complexes. These complexes activate and inhibit, respectively, follicle stimulating hormone secretion from the pituitary gland. The encoded protein also plays a role in eye, tooth and testis development. Elevated expression of this gene may be associated with cancer cachexia in human patients. [provided by RefSeq, Aug 2016]