Mouse IL-33 ELISA Kit | IL33 elisa kit

Mouse IL-33 PicoKine ELISA Kit

Cat. Num. AAA176553

• Gene Names: Il33; Il-33; Il1f11; NF-HEV; 9230117N10Rik
• Reactivity: Mouse
• Synonyms: IL-33; Mouse IL-33 PicoKine ELISA Kit; Interleukin-33; interleukin 33; C9orf26; DVS27; DVS27 related protein; IL-1F11; IL1F11; IL33; IL33_HUMAN; Interleukin-1 family member 11; NF-HEV; NFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2; IL33 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Il33
• Sequence Length: 266

• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL33 elisa kit

Principle of the Assay: The Picokine™ Mouse Il33 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Il33 with a 96-well strip plate that is pre-coated with antibody specific for Il33. The detection antibody is a biotinylated antibody specific for Il33. The capture antibody is polyclonal antibody from goat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Il33 with immunogen: Expression system for standard: E.coli; Immunogen sequence: S109-I266. The kit is analytically validated with ready-to-use reagents. To measure Mouse Il33, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The density of the yellow product is linearly proportional to Mouse Il33 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Il33 in the sample.

Background/Introduction: Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. By genomic sequence analysis, the IL33 gene is mapped to chromosome 9p24.1 and its mouse homolog to a syntenic region on chromosome 19qC1. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell (CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.

NCBI and Uniprot Product Information

• NCBI GI #: 68565902
• NCBI GeneID: 77125
• UniProt Accession #: Q8BVZ5; Q2YEJ4; Q3TQN0; Q99L46
• Molecular Weight: 29,991 Da
• NCBI Official Full Name: Interleukin-33
• NCBI Official Synonym Full Names: interleukin 33
• NCBI Official Symbol: Il33
• NCBI Official Synonym Symbols: Il-33; Il1f11; NF-HEV; 9230117N10Rik
• NCBI Protein Information: interleukin-33; nuclear factor from high endothelial venules
• UniProt Protein Name: Interleukin-33
• Protein Family: Interleukin
• UniProt Gene Name: Il33
• UniProt Synonym Gene Names: IL-33
• UniProt Entry Name: IL33_MOUSE

Uniprot Description

IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Cellular Component: extracellular space; extracellular region; chromosome; cytoplasmic vesicle; nucleus

Molecular Function: cytokine activity

Biological Process: positive regulation of T-helper 2 type immune response; negative regulation of T-helper 1 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; defense response to virus; positive regulation of interleukin-5 production; positive regulation of macrophage activation; positive regulation of inflammatory response