Rabbit anti-Human VAMP4 Polyclonal Antibody | anti-VAMP4 antibody

VAMP4, ID (VAMP4, Vesicle-associated membrane protein 4) (Azide free) (HRP)

Cat. Num. AAA6362195

• Gene Names: VAMP4; VAMP-4; VAMP24
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: VAMP4; Polyclonal Antibody; ID (VAMP4; Vesicle-associated membrane protein 4) (Azide free) (HRP); Vesicle-associated membrane protein 4; anti-VAMP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-VAMP4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: VAMP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the Central region of human VAMP4.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(VAMP4 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the VAMP4 antibody detected the VAMP4 protein (arrow).)

Related Product Information for anti-VAMP4 antibody

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. This protein may play a role in trans-Golgi network-to-endosome transport.

Product Categories/Family for anti-VAMP4 antibody

Antibodies; Membrane Proteins

References

Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Sabra, M.M., et al. Mol. Genet. Metab. 85(2):133-139(2005). Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004). Hinners, I., et al. EMBO Rep. 4(12):1182-1189(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 42544207
• NCBI GeneID: 8674
• NCBI Accession #: NP_003753.2
• NCBI GenBank Nucleotide #: NM_003762.4
• UniProt Accession #: O75379; Q96IY9; Q96J20; Q9UEL7; A2IDD8; Q6IAZ3
• Molecular Weight: 16,397 Da
• NCBI Official Full Name: vesicle-associated membrane protein 4 isoform 1
• NCBI Official Synonym Full Names: vesicle-associated membrane protein 4
• NCBI Official Symbol: VAMP4
• NCBI Official Synonym Symbols: VAMP-4; VAMP24
• NCBI Protein Information: vesicle-associated membrane protein 4; OTTHUMP00000190887; OTTHUMP00000190888; OTTHUMP00000233988
• UniProt Protein Name: Vesicle-associated membrane protein 4
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP4
• UniProt Synonym Gene Names: VAMP-4
• UniProt Entry Name: VAMP4_HUMAN

Uniprot Description

VAMP4: Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule. Belongs to the synaptobrevin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q24-q25

Cellular Component: SNARE complex; Golgi membrane; Golgi apparatus; cell surface; lysosome; integral to membrane; trans-Golgi network; endosome

Molecular Function: SNAP receptor activity; SNARE binding

Biological Process: regulation of Golgi to plasma membrane protein transport; ER to Golgi vesicle-mediated transport; exocytosis; Golgi to plasma membrane protein transport; microtubule cytoskeleton organization and biogenesis