Human Alanine Glyoxylate Aminotransferase 2 (AGXT2) ELISA Kit | AGXT2 elisa kit

Human Alanine Glyoxylate Aminotransferase 2 (AGXT2) ELISA Kit

Cat. Num. AAA2707650

• Gene Names: AGXT2; AGT2; BAIBA; DAIBAT
• Reactivity: Human
• Synonyms: Alanine Glyoxylate Aminotransferase 2 (AGXT2); Human Alanine Glyoxylate Aminotransferase 2 (AGXT2) ELISA Kit; AGT2; Beta-Alanine-Pyruvate Aminotransferase; (R)-3-amino-2-methylpropionate--pyruvate transaminase; AGXT2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Alanine Glyoxylate Aminotransferase 2 (AGXT2).; No significant cross-reactivity or interference between Alanine Glyoxylate Aminotransferase 2 (AGXT2) and analogues was observed.
• Sequence Length: 514

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 78-5,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 31pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alanine Glyoxylate Aminotransferase 2 (AGXT2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alanine Glyoxylate Aminotransferase 2 (AGXT2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGXT2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alanine Glyoxylate Aminotransferase 2 (AGXT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alanine Glyoxylate Aminotransferase 2 (AGXT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alanine Glyoxylate Aminotransferase 2 (AGXT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alanine Glyoxylate Aminotransferase 2 (AGXT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 17432913
• NCBI GeneID: 64902
• UniProt Accession #: Q9BYV1
• NCBI Official Full Name: Alanine--glyoxylate aminotransferase 2, mitochondrial
• NCBI Official Synonym Full Names: alanine--glyoxylate aminotransferase 2
• NCBI Official Symbol: AGXT2
• NCBI Official Synonym Symbols: AGT2; BAIBA; DAIBAT
• NCBI Protein Information: alanine--glyoxylate aminotransferase 2, mitochondrial
• UniProt Protein Name: Alanine--glyoxylate aminotransferase 2, mitochondrial
• Protein Family: Alanine--glyoxylate aminotransferase
• UniProt Gene Name: AGXT2
• UniProt Synonym Gene Names: AGT2; AGT 2
• UniProt Entry Name: AGT2_HUMAN

NCBI Description

The protein encoded by this gene is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. It is an important regulator of methylarginines and is involved in the control of blood pressure in kidney. Polymorphisms in this gene affect methylarginine and beta-aminoisobutyrate metabolism, and are associated with carotid atherosclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

AGXT2: is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. [provided by RefSeq, Dec 2008]

Protein type: EC 2.6.1.40; Mitochondrial; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.44; Transferase

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: beta-alanine-pyruvate transaminase activity; alanine-glyoxylate transaminase activity; (R)-3-amino-2-methylpropionate-pyruvate transaminase activity; pyridoxal phosphate binding

Biological Process: pyrimidine nucleoside catabolic process; positive regulation of nitric oxide biosynthetic process; glyoxylate catabolic process; pyrimidine base metabolic process; glyoxylate metabolic process; nucleobase, nucleoside and nucleotide metabolic process; glycine biosynthetic process, by transamination of glyoxylate; L-alanine catabolic process, by transamination