Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Monoclonal Antibody (SPM198).)
Mouse Mitochondria Monoclonal Antibody
Mitochondria (Marker for Human Cells) Mouse Monoclonal Antibody
Reactivity
Human.Does not react with Mouse and Rat
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Synonyms
Mitochondria; Monoclonal Antibody; Mitochondria (Marker for Human Cells) Mouse Monoclonal Antibody; anti-Mitochondria antibody
Host
Mouse
Reactivity
Human.
Does not react with Mouse and Rat
Does not react with Mouse and Rat
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SPM198
Specificity
This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Applicable Applications for anti-Mitochondria antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes
Immunofluorescence (0.5-1ug/ml)
Western Blot (0.25-0.5ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Western Blot (0.25-0.5ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Cellular Localization
Mitochondria in Cytoplasm
Immunogen
Semi-purified mitochondrial preparation
Positive Control
HeLa or HepG2 cells. Hepatic carcinoma.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody is stable for 24 months.,Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Monoclonal Antibody (SPM198).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Monoclonal Antibody (SPM198).)
References
Epstein, Alan L. and Clevenger, Charles V. In: Progress in Nonhistone Protein Research, Vol. 1, Isaac Bekhor, Ed. CRC Press, Boca Raton, FL, pp. 117-137, 1985
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Product Notes
The Mitochondria (Catalog #AAA438290) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mitochondria (Marker for Human Cells) Mouse Monoclonal Antibody reacts with Human. Does not react with Mouse and Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Mitochondria can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin. Immunofluorescence (0.5-1ug/ml) Western Blot (0.25-0.5ug/ml) Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the Mitochondria for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mitochondria, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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