Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with HSA Monoclonal Antibody (OCH1E5).)
Mouse anti-Human, Dog Hepatocyte Specific Antigen Monoclonal Antibody
Hepatocyte Specific Antigen (Hepatocellular Marker) Mouse Monoclonal Antibody
Reactivity
Human, Dog
Applications
Immunofluorescence, Immunohistochemistry
Synonyms
Hepatocyte Specific Antigen; Monoclonal Antibody; Hepatocyte Specific Antigen (Hepatocellular Marker) Mouse Monoclonal Antibody; anti-Hepatocyte Specific Antigen antibody
Host
Mouse
Reactivity
Human, Dog
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OCH1E5
Specificity
Hepatocyte Specific Antigen, also called Hepatocyte Paraffin 1 or HepPar1, localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCCs occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applicable Applications for anti-Hepatocyte Specific Antigen antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes
Immunofluorescence (0.5-1.0ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Cellular Localization
Finely granular Cytoplasmic
Immunogen
Extract of a formalin-fixed, rejected-allograft of a human liver
Positive Control
Liver or Hepatocellular Carcinoma (HCC)
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with HSA Monoclonal Antibody (OCH1E5).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with HSA Monoclonal Antibody (OCH1E5).)
References
Wennerberg AE et. al. Am J Pathol 1993;143:1050-4. Ramos-Vara, J.A., et al. Histochem 2002; J. 34: 397-401. Fan, Z., et al. Mod. Pathol 2003; 16: 137-144, 2003
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Product Notes
The Hepatocyte Specific Antigen (Catalog #AAA438219) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hepatocyte Specific Antigen (Hepatocellular Marker) Mouse Monoclonal Antibody reacts with Human, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Hepatocyte Specific Antigen can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin. Immunofluorescence (0.5-1.0ug/ml) Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the Hepatocyte Specific Antigen for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hepatocyte Specific Antigen, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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