Western Blot (WB)
((0.1ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Ugt2b Polyclonal Antibody | anti-Ugt2b antibody
Goat anti-Ugt2b (rat) (aa423-436) Antibody
Gene Names
Ugt2b; Ugt2b2
Reactivity
Tested: Rat; Expected from sequence similarity: Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Ugt2b; Polyclonal Antibody; Goat anti-Ugt2b (rat) (aa423-436) Antibody; UDP glycosyltransferase 2 family; polypeptide B; Ugt2b2; 3-hydroxyandrogen specific; 3-hydroxyandrogen-specific UDPGT; Androsterone UDP-glucuronosyltransferase; RLUG23; UDP glucuronosyltransferase; UDP-glucuronosyltransferase 2B2; UDPGT 2B2; UDPGTr; Ugt2b antibody; polypeptide B antibody; Ugt2b2 antibody; 3-hydroxyandrogen specific antibody; 3-hydroxyandrogen-specific UDPGT antibody; Androsterone UDP-glucuronosyltransferase antibody; RLUG23 antibody; UDP glucuronosyltransferase antibody; UDP-glucuronosyltransferase 2B2 antibody; UDPGTr antibody; Ugt2b (rat) (aa423-436); anti-Ugt2b antibody
Host
Goat
Reactivity
Tested: Rat; Expected from sequence similarity: Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KLDFLSALEEVIDN
Sequence Length
530
Applicable Applications for anti-Ugt2b antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 50kDa band observed in Rat Kidney lysates (calculated MW of 61. 0kDa according to NP_113721.3). Recommended concentration: 0.1-0.3ug/ml.
Western Blot: Approx 50kDa band observed in Rat Kidney lysates (calculated MW of 61. 0kDa according to NP_113721.3). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-KLDFLSALEEVIDN, from the internal region of the protein sequence according to NP_113721.3.
Epitope
Internal region
Note
This antibody is NOT expected to cross-react with other Ugt2 proteins
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.1ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,986 Da
NCBI Official Full Name
UDP-glucuronosyltransferase 2B2
NCBI Official Synonym Full Names
UDP glycosyltransferase 2 family, polypeptide B
NCBI Official Symbol
Ugt2b
NCBI Official Synonym Symbols
Ugt2b2
NCBI Protein Information
UDP-glucuronosyltransferase 2B2
UniProt Protein Name
UDP-glucuronosyltransferase 2B2
Protein Family
UniProt Gene Name
Ugt2b
UniProt Synonym Gene Names
Ugt2b2; UDPGT 2B2
UniProt Entry Name
UD2B2_RAT
NCBI Description
enzyme involved in conjugating lipophilic aglycon substrates with glucuronic acid [RGD, Feb 2006]
Uniprot Description
Ugt2b38: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. 2B2 acts on various endogenous steroids, especially etiocholanolone and androsterone
Cellular Component: endoplasmic reticulum membrane; integral to membrane; intracellular membrane-bound organelle
Molecular Function: glucuronosyltransferase activity; retinoic acid binding
Biological Process: flavonoid biosynthetic process
Research Articles on Ugt2b
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Product Notes
The Ugt2b ugt2b (Catalog #AAA422559) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Ugt2b (rat) (aa423-436) Antibody reacts with Tested: Rat; Expected from sequence similarity: Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ugt2b can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 50kDa band observed in Rat Kidney lysates (calculated MW of 61. 0kDa according to NP_113721.3). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the Ugt2b ugt2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KLDFLSALEE VIDN. It is sometimes possible for the material contained within the vial of "Ugt2b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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