Western Blot (WB)
((0.1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SWAP70 Polyclonal Antibody | anti-SWAP70 antibody
Goat anti-SWAP70 Antibody
Gene Names
SWAP70; HSPC321; SWAP-70
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
SWAP70; Polyclonal Antibody; Goat anti-SWAP70 Antibody; SWAP-70 protein; SWAP-70; KIAA0640; KIAA0640 protein; FLJ39540; HSPC321; SWAP switching B-cell complex 70kDa subunit; SWAP70 antibody; SWAP-70 protein antibody; SWAP-70 antibody; KIAA0640 antibody; KIAA0640 protein antibody; FLJ39540 antibody; HSPC321 antibody; SWAP switching B-cell complex 70kDa subunit antibody; anti-SWAP70 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EEREKNWKEKKTTE
Sequence Length
585
Applicable Applications for anti-SWAP70 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 70kDa band observed in Human Lymph Node and Human Peripheral Blood Mononucleocytes lysates (calculated MW of 69. 0kDa according to NP_055870.2). Recommended concentration: 0.1-0.3ug/ml.
Western Blot: Approx 70kDa band observed in Human Lymph Node and Human Peripheral Blood Mononucleocytes lysates (calculated MW of 69. 0kDa according to NP_055870.2). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-EEREKNWKEKKTTE, from the C Terminus of the protein sequence according to NP_055870.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,998 Da
NCBI Official Full Name
switch-associated protein 70 isoform 1
NCBI Official Synonym Full Names
SWAP switching B-cell complex 70kDa subunit
NCBI Official Symbol
SWAP70
NCBI Official Synonym Symbols
HSPC321; SWAP-70
NCBI Protein Information
switch-associated protein 70
UniProt Protein Name
Switch-associated protein 70
Protein Family
UniProt Gene Name
SWAP70
UniProt Synonym Gene Names
KIAA0640; SWAP-70
UniProt Entry Name
SWP70_HUMAN
Uniprot Description
SWAP-70: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
Protein type: GEFs; GEFs, Rac/Rho
Chromosomal Location of Human Ortholog: 11p15
Cellular Component: cytoskeleton; cytosol; lamellipodium; nucleus; plasma membrane
Molecular Function: ATP binding; calcium ion binding; DNA binding; protein binding
Biological Process: actin filament bundle formation; elevation of cytosolic calcium ion concentration; isotype switching; negative regulation of actin filament depolymerization; negative regulation of cell-cell adhesion mediated by integrin; positive regulation of actin filament bundle formation; regulation of protein localization
Research Articles on SWAP70
Similar Products
Product Notes
The SWAP70 swap70 (Catalog #AAA420747) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SWAP70 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SWAP70 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 70kDa band observed in Human Lymph Node and Human Peripheral Blood Mononucleocytes lysates (calculated MW of 69. 0kDa according to NP_055870.2). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the SWAP70 swap70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EEREKNWKEK KTTE. It is sometimes possible for the material contained within the vial of "SWAP70, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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