Goat TCL1A Polyclonal Antibody | anti-TCL1A antibody

Goat anti-TCL1A Antibody

Cat. Num. AAA420412

• Gene Names: TCL1A; TCL1
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TCL1A; Polyclonal Antibody; Goat anti-TCL1A Antibody; T-cell leukemia/lymphoma 1A; TCL1; T-cell lymphoma-1; T-cell lymphoma-1A; TCL1A antibody; T-cell leukemia/lymphoma 1A antibody; TCL1 antibody; T-cell lymphoma-1 antibody; T-cell lymphoma-1A antibody; anti-TCL1A antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: QLYPDGRYRSSD
• Sequence Length: 114

• Applicable Applications for anti-TCL1A antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Approx 16kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 13.5kDa according to NP_068801.1). Recommended concentration: 0.1-0.3ug/ml.
• Immunogen: Peptide with sequence C-QLYPDGRYRSSD, from the internal region of the protein sequence according to NP_068801.1.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Jurkat Cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 11415028
• NCBI GeneID: 8115
• NCBI Accession #: NP_068801.1
• NCBI GenBank Nucleotide #: NM_021966.2
• Molecular Weight: 13,460 Da
• NCBI Official Full Name: T-cell leukemia/lymphoma protein 1A
• NCBI Official Synonym Full Names: T-cell leukemia/lymphoma 1A
• NCBI Official Symbol: TCL1A
• NCBI Official Synonym Symbols: TCL1
• NCBI Protein Information: T-cell leukemia/lymphoma protein 1A
• UniProt Protein Name: T-cell leukemia/lymphoma protein 1A
• Protein Family: T-cell leukemia/lymphoma protein
• UniProt Gene Name: TCL1A
• UniProt Synonym Gene Names: TCL1; Oncogene TCL1
• UniProt Entry Name: TCL1A_HUMAN

NCBI Description

Overexpression of the TCL1 gene in humans has been implicated in the development of mature T cell leukemia, in which chromosomal rearrangements bring the TCL1 gene in close proximity to the T-cell antigen receptor (TCR)-alpha (MIM 186880) or TCR-beta (MIM 186930) regulatory elements (summarized by Virgilio et al., 1998 [PubMed 9520462]). In normal T cells TCL1 is expressed in CD4-/CD8- cells, but not in cells at later stages of differentiation. TCL1 functions as a coactivator of the cell survival kinase AKT (MIM 164730) (Laine et al., 2000 [PubMed 10983986]).[supplied by OMIM, Jul 2010]

Uniprot Description

TCL1A: Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. Homodimer. Interacts with AKT1, AKT2 and AKT3 (via PH domain). Interacts with PNPT1; the interaction has no effect on PNPT1 exonuclease activity. Restricted in the T-cell lineage to immature thymocytes and activated peripheral lymphocytes. Preferentially expressed early in T- and B-lymphocyte differentiation. Belongs to the TCL1 family.

Protein type: Protein kinase, regulatory subunit; Oncoprotein

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: endoplasmic reticulum; nucleus

Molecular Function: protein binding

Biological Process: multicellular organismal development