Mouse Myosin VA (MYO5A) ELISA Kit | MYO5A elisa kit

Mouse Myosin VA (MYO5A) ELISA Kit

Cat. Num. AAA2705111

• Gene Names: MYO5A; GS1; MYO5; MYH12; MYR12
• Reactivity: Mouse
• Synonyms: Myosin VA (MYO5A); Mouse Myosin VA (MYO5A) ELISA Kit; GS1; MYH12; MYO5; MYOXIN; MYR12; myosin V; myosin Va; Myosin; Heavy Polypeptide Kinase; Myosin Heavy Chain 12; Myoxin; Myosin V; Dilute myosin heavy chain; non-muscle; MYO5A elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin VA (MYO5A).; No significant cross-reactivity or interference between Myosin VA (MYO5A) and analogues was observed.
• Sequence Length: 1,880

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.045ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin VA (MYO5A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin VA (MYO5A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYO5A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin VA (MYO5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin VA (MYO5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin VA (MYO5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin VA (MYO5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 3064064
• NCBI GeneID: 4644
• UniProt Accession #: Q9Y4I1; O60653; Q07902; Q16249; Q9UE30; Q9UE31; A8MZC5
• Molecular Weight: 218,608 Da
• NCBI Official Full Name: myosin Va, partial
• NCBI Official Synonym Full Names: myosin VA
• NCBI Official Symbol: MYO5A
• NCBI Official Synonym Symbols: GS1; MYO5; MYH12; MYR12
• NCBI Protein Information: unconventional myosin-Va
• UniProt Protein Name: Unconventional myosin-Va
• Protein Family: Unconventional myosin
• UniProt Gene Name: MYO5A
• UniProt Synonym Gene Names: MYH12

NCBI Description

This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq, Dec 2008]

Uniprot Description

Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.