Western Blot (WB)
((0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Ramp1 Polyclonal Antibody | anti-Ramp1 antibody
Goat anti-Ramp1 (C-Term., mouse) Antibody
Gene Names
Ramp1; 9130218E19Rik
Reactivity
Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Ramp1; Polyclonal Antibody; Goat anti-Ramp1 (C-Term.; mouse) Antibody; receptor (calcitonin) activity modifying protein 1; 9130218E19Rik; MGC38240; receptor-activity modifying protein 1; Ramp1 antibody; receptor (calcitonin) activity modifying protein 1 antibody; MGC38240 antibody; receptor-activity modifying protein 1 antibody; Ramp1 (C-Term.; mouse); anti-Ramp1 antibody
Host
Goat
Reactivity
Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
DYQSKRTEGIV
Sequence Length
148
Applicable Applications for anti-Ramp1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Experiments gave bands at approx.18kDa and 40kDa in Rat Brain lysate after 0.3ug/ml antibody staining. The appearance of a band with much higher molecular weight corresponds to earlier findings in literature with different antibodies (Hilairet, J Biol Chem.2001 Nov 9;276(45): 42182-90.; PMID: 11535606). This protein has a calculated MW of 16.9kDa according to Mouse NP_058590.1 and 16.8kDa according to Rat NP_113833.1. Recommended concentration: 0.3-1ug/ml.
Western Blot: Experiments gave bands at approx.18kDa and 40kDa in Rat Brain lysate after 0.3ug/ml antibody staining. The appearance of a band with much higher molecular weight corresponds to earlier findings in literature with different antibodies (Hilairet, J Biol Chem.2001 Nov 9;276(45): 42182-90.; PMID: 11535606). This protein has a calculated MW of 16.9kDa according to Mouse NP_058590.1 and 16.8kDa according to Rat NP_113833.1. Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-DYQSKRTEGIV, from the C Terminus of the protein sequence according to NP_058590.1; NP_848488.2.
Epitope
C Terminus
Note
This antibody is expected to recognize isoform 1 (NP_058590.1) only.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,892 Da
NCBI Official Full Name
receptor activity-modifying protein 1 isoform 1
NCBI Official Synonym Full Names
receptor (calcitonin) activity modifying protein 1
NCBI Official Symbol
Ramp1
NCBI Official Synonym Symbols
9130218E19Rik
NCBI Protein Information
receptor activity-modifying protein 1
UniProt Protein Name
Receptor activity-modifying protein 1
Protein Family
UniProt Gene Name
Ramp1
UniProt Entry Name
RAMP1_MOUSE
Uniprot Description
RAMP1: Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL. Belongs to the RAMP family.
Protein type: Receptor, GPCR; Membrane protein, integral
Cellular Component: cell surface; extracellular space; integral to membrane; membrane; plasma membrane; receptor complex
Molecular Function: calcitonin receptor activity; calcitonin receptor binding; coreceptor activity; protein transporter activity; receptor activity
Biological Process: angiogenesis; calcium ion transport; G-protein coupled receptor protein signaling pathway; intracellular protein transport; positive regulation of cAMP biosynthetic process; positive regulation of protein amino acid glycosylation; protein transport; receptor internalization; regulation of G-protein coupled receptor protein signaling pathway; transport
Research Articles on Ramp1
Similar Products
Product Notes
The Ramp1 ramp1 (Catalog #AAA421948) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Ramp1 (C-Term., mouse) Antibody reacts with Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ramp1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Experiments gave bands at approx.18kDa and 40kDa in Rat Brain lysate after 0.3ug/ml antibody staining. The appearance of a band with much higher molecular weight corresponds to earlier findings in literature with different antibodies (Hilairet, J Biol Chem.2001 Nov 9;276(45): 42182-90.; PMID: 11535606). This protein has a calculated MW of 16.9kDa according to Mouse NP_058590.1 and 16.8kDa according to Rat NP_113833.1. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the Ramp1 ramp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DYQSKRTEGI V. It is sometimes possible for the material contained within the vial of "Ramp1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
