Western Blot (WB)
((0.2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat RAB11FIP1/RCP Polyclonal Antibody | anti-RAB11FIP1 antibody
Goat anti-RAB11FIP1/RCP Antibody
Western Blot: Approx 80kDa band observed in lysates of cell line HeLa (calculated MW of 71. 0kDa according to NP_079427.3). The observed molecular weight of 80kDa corresponds to earlier findings in literature with different antibodies (Lindsay et al, J Biol Chem.2002 Apr 5;277(14): 12190-9. PMID 11786538. ). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of the Rab11-family interacting proteins (Rab11-FIPs), which play a role in the Rab-11 mediated recycling of vesicles. The encoded protein may be involved in endocytic sorting, trafficking of proteins including integrin subunits and epidermal growth factor receptor (EGFR), and transport between the recycling endosome and the trans-Golgi network. Alternative splicing results in multiple transcript variants. A pseudogene is described on the X chromosome. [provided by RefSeq, Dec 2013]
Uniprot Description
RAB11FIP1: A Rab11 effector protein involved in the endosomal recycling process. Also involved in controlling membrane trafficking along the phagocytic pathway and in phagocytosis. Homooligomer (isoform 2). Isoform 2 interacts with RAB4A, RAB11A, RAB11B and RAB25. According to PubMed:15280022, RAB4A binding to RAB11FIP1 is of very low affinity in vitro and in vivo. Isoform 2 is expressed in brain, heart, testis, lung, spleen, ovary and small intestine. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 8p11.22
Cellular Component: cytoplasm; intracellular membrane-bound organelle; phagocytic vesicle membrane; recycling endosome
Molecular Function: Rab GTPase binding
Biological Process: protein transport; regulated secretory pathway
Research Articles on RAB11FIP1
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Product Notes
The RAB11FIP1 rab11fip1 (Catalog #AAA420243) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RAB11FIP1/RCP Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAB11FIP1/RCP can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 80kDa band observed in lysates of cell line HeLa (calculated MW of 71. 0kDa according to NP_079427.3). The observed molecular weight of 80kDa corresponds to earlier findings in literature with different antibodies (Lindsay et al, J Biol Chem.2002 Apr 5;277(14): 12190-9. PMID 11786538.). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the RAB11FIP1 rab11fip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EATKEAKESK KPE. It is sometimes possible for the material contained within the vial of "RAB11FIP1/RCP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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