Goat FCER1A Polyclonal Antibody | anti-FCER1A antibody

Goat anti-FCER1A (aa177-189) Antibody

Cat. Num. AAA423275

• Gene Names: FCER1A; FCE1A; FcERI
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: FCER1A; Polyclonal Antibody; Goat anti-FCER1A (aa177-189) Antibody; Fc fragment of IgE; high affinity I; receptor for; alpha polypeptide; FCE1A; FcERI; Fc IgE receptor; Fc epsilon RI alpha-chain; Fc-epsilon RI-alpha; high affinity immunoglobulin epsilon receptor alpha-subunit; high affinity immu; FCER1A antibody; receptor for antibody; alpha polypeptide antibody; FCE1A antibody; FcERI antibody; Fc epsilon RI alpha-chain antibody; Fc-epsilon RI-alpha antibody; high affinity immunoglobulin epsilon receptor alpha-subunit antibody; high affinity immunoglobulin epsilon receptor subunit alpha antibody; igE Fc receptor subunit alpha antibody; immunoglobulin E receptor; high-affinity; of mast cells; FCER1A (aa177-189); anti-FCER1A antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: TGKVWQLDYESEP
• Sequence Length: 257

• Applicable Applications for anti-FCER1A antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Approx 50kDa band observed in Human Pripheral Blood Mononucleocytes lysates (calculated MW of 29.6kDa according to NP_001992.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ganguly et al, J Biol Chem.2007 Nov 9;282(45): 32758-64. PMID: 17728245). Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence C-TGKVWQLDYESEP, from the internal region of the protein sequence according to NP_001992.1.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Human Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4503675
• NCBI GeneID: 2205
• NCBI Accession #: NP_001992.1
• NCBI GenBank Nucleotide #: NM_002001.3
• Molecular Weight: 29,596 Da
• NCBI Official Full Name: high affinity immunoglobulin epsilon receptor subunit alpha
• NCBI Official Synonym Full Names: Fc fragment of IgE receptor Ia
• NCBI Official Symbol: FCER1A
• NCBI Official Synonym Symbols: FCE1A; FcERI
• NCBI Protein Information: high affinity immunoglobulin epsilon receptor subunit alpha
• UniProt Protein Name: High affinity immunoglobulin epsilon receptor subunit alpha
• Protein Family: High affinity immunoglobulin epsilon receptor
• UniProt Gene Name: FCER1A
• UniProt Synonym Gene Names: FCE1A; FcERI
• UniProt Entry Name: FCERA_HUMAN

NCBI Description

The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]

Uniprot Description

Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.