Western Blot (WB)
((0.1ug/ml) staining of fetal Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Nat8l Polyclonal Antibody | anti-Nat8l antibody
Goat anti-Nat8l (mouse aa211-223) Antibody
Gene Names
Nat8l; Shati; 1110038O08Rik
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Nat8l; Polyclonal Antibody; Goat anti-Nat8l (mouse aa211-223) Antibody; N-acetyltransferase 8-like; 1110038O08Rik; Shati; N-acetylaspartate synthetase; N-acetyltransferase 8-like protein; NAA synthetase; protein Shati; 1110038O08Rik antibody; NAA synthetase antibody; N-acetylaspartate synthetase antibody; N-acetyltransferase 8-like antibody; N-acetyltransferase 8-like protein antibody; Nat8l antibody; protein Shati antibody; Shati antibody; Nat8l (mouse aa211-223); anti-Nat8l antibody
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SVDSRFRGKGIAK
Sequence Length
299
Applicable Applications for anti-Nat8l antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 45kDa band observed in fetal Mouse Brain lysates (calculated MW of 32.7kDa according to NP_001001985.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Becker et al, J Biol Chem.2010 Sep 17;285(38): 29156-64. PMID: 20643647). Recommended concentration: 0.1-0.3ug/ml.
Western Blot: Approx 45kDa band observed in fetal Mouse Brain lysates (calculated MW of 32.7kDa according to NP_001001985.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Becker et al, J Biol Chem.2010 Sep 17;285(38): 29156-64. PMID: 20643647). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-SVDSRFRGKGIAK, from the internal region of the protein sequence according to NP_001001985.3.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.1ug/ml) staining of fetal Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of fetal Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,777 Da
NCBI Official Full Name
N-acetylaspartate synthetase
NCBI Official Synonym Full Names
N-acetyltransferase 8-like
NCBI Official Symbol
Nat8l
NCBI Official Synonym Symbols
Shati; 1110038O08Rik
NCBI Protein Information
N-acetylaspartate synthetase
UniProt Protein Name
N-acetylaspartate synthetase
Protein Family
UniProt Gene Name
Nat8l
UniProt Synonym Gene Names
NAA synthetase
UniProt Entry Name
NAT8L_MOUSE
Uniprot Description
NAT8L: Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake. Defects in NAT8L are the cause of N-acetylaspartate deficiency (NACED). A metabolic disorder resulting in truncal ataxia, marked developmental delay, seizures, and secondary microcephaly. Belongs to the camello family.
Protein type: Membrane protein, integral; Acetyltransferase; EC 2.3.1.17
Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; intracellular membrane-bound organelle; membrane; mitochondrial membrane; mitochondrion
Molecular Function: aspartate N-acetyltransferase activity; N-acetyltransferase activity; transferase activity; transferase activity, transferring acyl groups
Biological Process: positive regulation of dopamine uptake
Research Articles on Nat8l
Similar Products
Product Notes
The Nat8l nat8l (Catalog #AAA423079) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Nat8l (mouse aa211-223) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Nat8l can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 45kDa band observed in fetal Mouse Brain lysates (calculated MW of 32.7kDa according to NP_001001985.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Becker et al, J Biol Chem.2010 Sep 17;285(38): 29156-64. PMID: 20643647). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the Nat8l nat8l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SVDSRFRGKG IAK. It is sometimes possible for the material contained within the vial of "Nat8l, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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