Western Blot (WB)
((0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PGRP-S Polyclonal Antibody | anti-PGLYRP1 antibody
Goat anti-PGRP-S Antibody
Gene Names
PGLYRP1; PGRP; TAG7; PGRPS; PGLYRP; PGRP-S; TNFSF3L
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PGRP-S; Polyclonal Antibody; Goat anti-PGRP-S Antibody; PGRPS; PGLYRP1; peptidoglycan recognition protein 1; MGC126894; MGC126896; PGLYRP; PGRP; TAG7; TNFSF3L; TNF superfamily; member 3 (LTB)-like (peptidoglycan recognition protein); PGRP-S antibody; PGRPS antibody; PGLYRP1 antibody; peptidoglycan recognition protein 1 antibody; MGC126894 antibody; MGC126896 antibody; PGLYRP antibody; PGRP antibody; TAG7 antibody; TNFSF3L antibody; member 3 (LTB)-like (peptidoglycan recognition protein) antibody; anti-PGLYRP1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
PGNQLYHLIQN
Sequence Length
196
Applicable Applications for anti-PGLYRP1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Immunohistochemistry: In paraffin embedded Human Spleen shows staining of select splenocytes. Recommended concentration, 3-6ug/ml.
Western Blot: Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 21.7kDa according to NP_005082.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lu et al, J Biol Chem.2006 Mar 3;281(9): 5895-907; PMID: 16354652). Recommended concentration: 0.3-1ug/ml.
Immunohistochemistry: In paraffin embedded Human Spleen shows staining of select splenocytes. Recommended concentration, 3-6ug/ml.
Western Blot: Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 21.7kDa according to NP_005082.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lu et al, J Biol Chem.2006 Mar 3;281(9): 5895-907; PMID: 16354652). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-PGNQLYHLIQN, from the C Terminus of the protein sequence according to NP_005082.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,731 Da
NCBI Official Full Name
peptidoglycan recognition protein 1
NCBI Official Synonym Full Names
peptidoglycan recognition protein 1
NCBI Official Symbol
PGLYRP1
NCBI Official Synonym Symbols
PGRP; TAG7; PGRPS; PGLYRP; PGRP-S; TNFSF3L
NCBI Protein Information
peptidoglycan recognition protein 1
UniProt Protein Name
Peptidoglycan recognition protein 1
Protein Family
UniProt Gene Name
PGLYRP1
UniProt Synonym Gene Names
PGLYRP; PGRP; TNFSF3L; PGRP-S
UniProt Entry Name
PGRP1_HUMAN
Uniprot Description
PGLYRP1: Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram- negative bacteria, and has bacteriostatic activity towards Gram- negative bacteria. Plays a role in innate immunity. Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.
Protein type: Secreted; Receptor, misc.; Apoptosis; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 19q13.2-q13.3
Cellular Component: extracellular region
Molecular Function: N-acetylmuramoyl-L-alanine amidase activity; peptidoglycan binding; peptidoglycan receptor activity; zinc ion binding
Biological Process: defense response to Gram-positive bacterium; detection of bacterium; immune response; innate immune response; negative regulation of inflammatory response; negative regulation of interferon-gamma production; negative regulation of natural killer cell differentiation during immune response; pattern recognition receptor signaling pathway; peptidoglycan catabolic process
Research Articles on PGLYRP1
Similar Products
Product Notes
The PGLYRP1 pglyrp1 (Catalog #AAA421952) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PGRP-S Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's PGRP-S can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 4000. Immunohistochemistry: In paraffin embedded Human Spleen shows staining of select splenocytes. Recommended concentration, 3-6ug/ml. Western Blot: Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 21.7kDa according to NP_005082.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lu et al, J Biol Chem.2006 Mar 3;281(9): 5895-907; PMID: 16354652). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the PGLYRP1 pglyrp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PGNQLYHLIQ N. It is sometimes possible for the material contained within the vial of "PGRP-S, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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