Western Blot (WB)
((0. 01ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat HCAP-H Polyclonal Antibody | anti-NCAPH antibody
Goat anti-HCAP-H Antibody
Western Blot: Approx 110kDa band observed in lysates of cell line K562 (calculated MW of 82.6kDa according to NP_056156.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cabello et al, Mol Biol Cell.2001 Nov;12(11): 3527-37.; PMID: 11694586). Recommended concentration: 0.01-0.03ug/ml.
Western Blot (WB)
((0. 01ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described. [provided by RefSeq, Jul 2013]
Uniprot Description
NCAPH: Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Belongs to the CND2 (condensin subunit 2) family.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 2q11.2
Cellular Component: condensin complex; cytosol; membrane; nucleus
Molecular Function: protein binding
Biological Process: cell division; mitotic chromosome condensation
Research Articles on NCAPH
Similar Products
Product Notes
The NCAPH ncaph (Catalog #AAA421115) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-HCAP-H Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's HCAP-H can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 110kDa band observed in lysates of cell line K562 (calculated MW of 82.6kDa according to NP_056156.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cabello et al, Mol Biol Cell.2001 Nov;12(11): 3527-37.; PMID: 11694586). Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the NCAPH ncaph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TEDLSDVLVR QGD. It is sometimes possible for the material contained within the vial of "HCAP-H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
