Rabbit anti-Human ACE-1 (Angiotension Converting Enzyme, Angiotension I-Converting Enzyme, Peptidyl-Dipeptidase-A) Polyclonal Antibody | anti-ACE-1 antibody

Anti-ACE-1 (Angiotension Converting Enzyme, Angiotension I-Converting Enzyme, Peptidyl-dipeptidase-A), Amino Catalytic Domain Antibody

Cat. Num. AAA4157497

• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: ACE-1 (Angiotension Converting Enzyme; Angiotension I-Converting Enzyme; Peptidyl-Dipeptidase-A); Polyclonal Antibody; Anti-ACE-1 (Angiotension Converting Enzyme; Peptidyl-dipeptidase-A); Amino Catalytic Domain Antibody; anti-ACE-1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The antibody recognizes metallopeptidase ACE1.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-ACE-1 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Predicted molecular weight: 150 kDa. Dilution optimised using Chromogenic detection.
• Immunogen: The antibody is made to a synthetic peptide based on the first peptidase unit of human ACE1 (Human).
• Cellular Localization: Type I Membrane Protein
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-ACE-1 antibody

Description: The antibody is a polyclonal antibody made to the metallopeptidase ACE-1. The antibody is made to a synthetic peptide based on the first peptidase unit of human ACE-1. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant. Angiotension Converting Enzyme is a metallopeptidase of the MA(E) subclan of peptidases, also know as the-Glu-zincins-which use the motif HExxH as a catalytic triad. ACE-1 contains two catalytic domains, each domain using the HHEMGH zinc binding catalytic site. Although the catalytic domains (peptidase units 1 and 2) are similar in sequence, and are both catalytically active, they show some substrate specificity differences, and specific inhibitors have been crafted for each of the catalytic domains. In addition to the double peptidase form of ACE-1 (known as somatic ACE, or sACE), there is a testis form that lacks the first catalytic domain, and is referred to as germinal ACE, or gACE. The gACE-1 sequence arises from addition of a second promoter in intron 12 of ACE-1, and the first 72 residues are intron derived. ACE-1 is a type-II transmembrane protein, and the cytoplasmic domain has been shown to be a site of phospho-regulation. A form of ACE-1 lacking the transmembrane region exists, and may be a constitutively soluble form of the enzyme. The stalk region that separates the transmembrane region from the catalytic domain(s) is the cleavage site of a sheddase that releases the catalytic domains of full-length ACE-1 from the cell surface. ACE-1 is best known as a carboxy dipeptidase that cleaves the two carboxyterminal amino acids from Angiotension I, converting it to Angiotension II. ACE-1 is found in the lung, kidney, heart, pancreas, prostate, testis, small intestine and colon, and is found in cell lysates and as shed or processed forms in cell culture media and in biological fluids. The full-length sequence for sACE-1 encodes a 1306 amino acid protein with predicted mass of 149.7 kDa and a pI of 5.93. Glycosylation and other post-translational modifications make the apparent molecular weight on SDS PAGE gels larger, 175-184 kDa by Western blot. The shed forms range in size from 175-130 kDa. Many smaller forms may be seen on Western blot of culture media and biological fluid. The germinal form is 739 amino acids, with predicted mass of 84 kDa and pI of 5.87, and the soluble form lacking the TM region is 732 amino acids, with predicted mass of 83.3 kDa and a pI of 6.13. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.

Relevance: ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.