Rabbit anti-Human ACE-2 (Angiotension-Converting Enzyme 2, Angiotension-Converting Enzyme Homolog (ACEH) Polyclonal Antibody | anti-ACE-2 antibody

Anti-ACE-2 (Angiotension-Converting Enzyme 2, Angiotension-Converting Enzyme Homolog (ACEH), Carboxy End of Catalytic Domain Antibody

Cat. Num. AAA4157502

• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: ACE-2 (Angiotension-Converting Enzyme 2; Angiotension-Converting Enzyme Homolog (ACEH); Polyclonal Antibody; Anti-ACE-2 (Angiotension-Converting Enzyme 2; Carboxy End of Catalytic Domain Antibody; anti-ACE-2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The antibody recognises the Carboxy end of Catalytic domain of Angiotensin Converting Enzyme 2.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-ACE-2 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. The full-length sequence for ACE-2 encodes an 805 amino acid protein with predicted mass of 92kDa. However,glycosylation and other post-translational modifications make the apparent molecular weight on SDS PAGE gels larger, about 120 kDa by Western blot. Many smaller forms may be seen on Western blot of culture media and biological fluid. Dilution optimised using Chromogenic detection.
• Immunogen: Synthetic peptide corresponding to the carboxy end of the catalytic domain of Human Angiotensin Converting Enzyme 2, between the zinc binding site and the sheddase cleavage site
• Cellular Localization: Cell Membrane; Single Pass Type I Membrane Protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-ACE-2 antibody

Description: The antibody is a polyclonal antibody made to the metallopeptidase ACE-2. The antibody is made to a synthetic peptide based on the carboxy end of the catalytic domain of human ACE-2, between the zinc binding site and the sheddase cleavage site. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant. Angiotension Converting Enzyme 2 is a metallopeptidase of the MA(E) subclan of peptidases, also know as the-Glu-zincins-which use the motif HExxH as a catalytic triad. ACE-2 shares the HHEMGH catalytic domain with ACE-1, but otherwise homology is low between ACE-1 and ACE-2 (40% identity). ACE-2 is a type-II transmembrane protein, like ACE-1, and contains one catalytic domain, making it more similar to the germinal form of ACE-1 than the somatic, long form. The stalk region that separates the transmembrane region from the catalytic domain(s) is the cleavage site of a sheddase that releases the catalytic domains of full-length ACE-2 from the cell surface. ACE-2 differs from ACE-1 in enzymatic activity. Whereas ACE-1 is a carboxy dipeptidase that cleaves Angiotension I to form Angiotension II, ACE-2 cleaves Angiotension II to form Angiotension (1-7), an action that would downregulate the Angiotension II activity. ACE-1 inhibitors such as catopril and lisinopril do not block ACE-2 activity. ACE-1 is found in the lung, kidney, heart, pancreas, prostate, testis, small intestine and colon, and is found in cell lysates and as shed or processed forms in cell culture media and in biological fluids, but ACE-2 is more restricted to the testis, heart, kidney and colon. The full-length sequence for ACE-2 encodes an 805 amino acid protein with predicted mass of 92.46 kDa and a pI of 5.22. Glycosylation and other post-translational modifications make the apparent molecular weight on SDS PAGE gels larger, about 120 kDa by Western blot. Many smaller forms may be seen on Western blot of culture media and biological fluid. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.

Relevance: Angiotensin converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1 7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2-mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function. ACE1 inhibitors such as catopril and lisinopril do not block ACE2 activity. ACE2 is restricted to the testis, heart, kidney and colon. The full length sequence for ACE2 encodes an 805 amino acid protein with predicted mass of 92.46 kDa and a pI of 5.22.