Rabbit anti-Human HAI-1 Polyclonal Antibody | anti-HAI-1 antibody

Anti-HAI-1 (HGFA (Hepatocyte Growth Factor Activator) Inhibitor-1), Cytoplasmic Domain Antibody

Cat. Num. AAA4157560

• Gene Names: SPINT1; HAI; HAI1; MANSC2
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: HAI-1; Polyclonal Antibody; Anti-HAI-1 (HGFA (Hepatocyte Growth Factor Activator) Inhibitor-1); Cytoplasmic Domain Antibody; anti-HAI-1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is specific for HGFA Inhibitor 1 and HAI-1B (a splice variant).
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 529

• Applicable Applications for anti-HAI-1 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Detects bands of approximately 58kDa and 40kDa (predicted molecular weight: 58 kDa). Dilution optimised using Chromogenic detection.
• Immunogen: Synthetic peptide based on the cytoplasmic domain of human HGFA Inhibitor 1 sequence.
• Cellular Localization: Cell Membrane and Secreted
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-HAI-1 antibody

Description: The antibody is a rabbit polyclonal antibody made to HAI-1. The antibody is made to a synthetic peptide based on the cytoplasmic domain of human sequence, and will recognize HAI-1 and HAI-1B. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant. HAI-1 is an endogenous inhibitor of the kunitz-type serine proteinase HGF-activator (HGFA). HGF was first described as a hepatocyte-specific mitogen and survival factor, and has since been shown to exert a variety of actions on many cell types by binding to its MET receptor. HGF is activated by cleavage of the single-chain form to form a two-chain version by HGFA. HAI-1 also inhibits trypsin, and MTSP-1 (Matriptase), a serine proteinase that also activates HGF. HAI-1B is a splice variant of HAI-1, and contains a 16 amino acid insert after the first kringle domain, relative to the HAI-1 sequence. The longer sequence (HAI-1B) is 529 amino acids in length, with a predicted molecular weight of 58.4 kDa and a pI of 5.832. The shorter sequence, with 86% identity to HAI-1B, is 513 amino acids long, predicted weight of 56.9 kDa and a pI of 5.724. Message for each form is found in the same cells in roughly the same levels, but there does seem to be some differences in activity in the two forms. HAI-1 is more distantly related to HAI-2, which is found in 252 and 106 amino acid forms, predicting 35.4 and 10.9 kDa respectively, and more basic pIs of 8.09 and 8.639. The HAI proteins are glycosylated, and may have other post-translational modifications, thus the apparent molecular weight is 66 kDa for the full-length HAI-1. The HAI-1 antibodies do not recognize HAI-2. HAI-1 is down regulated in some tumor cells while MTSP-1 and HGFA levels are elevated, yielding increased net activity for these two HGF activators. In other cases, especially in tissues surrounding tumors, HAI-1 levels are thought to be elevated, perhaps in a protective stance. HAI-1 is secreted and membrane-inserted via a type-I transmembrane domain. The carboxyterminal end is a cytoplasmic domain, thought to interact with signaling and cytoskeletal machinery. The extracellular domains include a kringle domain (KD1) followed by an LDL receptor-like motif, another kringle domain (KD2), which is thought to be inactive or with much lower activity against HGF. HAI-1 is shed from the cell surface by cleavage extracellular to the transmembrane domain, yielding a 58 kDa form, and cleavage between the KD1 and the LDLR motif yields a 39-40 kDa form, which is thought to be more proteolytically active. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.

Relevance: HGFA Inhibitor 1 is an endogenous inhibitor of the kunitz-type serine proteinase HGF-activator (HGFA). HGF was first described as a hepatocyte-specific mitogen and survival factor, and has since been shown to exert a variety of actions on many cell types by binding to its MET receptor. HGF is activated by cleavage of the single-chain form to form a two-chain version by HGFA. HGFA Inhibitor 1 also inhibits trypsin, and MTSP-1 (Matriptase), a serine proteinase that also activates HGF. HGFB Inhibitor 1 is a splice variant of HGFA Inhibitor 1, and contains a 16 amino acid insert after the first kringle domain, relative to the HGFA Inhibitor 1 sequence.

NCBI and Uniprot Product Information

• NCBI GI #: 37183056
• NCBI GeneID: 6692
• NCBI Official Full Name: HAI-1
• NCBI Official Synonym Full Names: serine peptidase inhibitor, Kunitz type 1
• NCBI Official Symbol: SPINT1
• NCBI Official Synonym Symbols: HAI; HAI1; MANSC2
• NCBI Protein Information: kunitz-type protease inhibitor 1

NCBI Description

The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]