Western Blot (WB)
((0.5ug/ml) staining of Mouse Small Intestine lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Slc10a2 Polyclonal Antibody | anti-Slc10a2 antibody
Goat anti-Slc10a2 (mouse) Antibody
Gene Names
Slc10a2; ASBT; ISBT
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Slc10a2; Polyclonal Antibody; Goat anti-Slc10a2 (mouse) Antibody; solute carrier family 10 (sodium/bile acid cotransporter family); member 2; ASBT; ISBT; ileal apical sodium-dependent bile acid transporter; ileal sodium-dependent bile acid transporter; solute carrier family 10; Slc10a2 antibody; member 2 antibody; ASBT antibody; ISBT antibody; ileal apical sodium-dependent bile acid transporter antibody; ileal sodium-dependent bile acid transporter antibody; Slc10a2 (mouse); anti-Slc10a2 antibody
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
DETNKGFQPDEK
Sequence Length
348
Applicable Applications for anti-Slc10a2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 35kDa band observed in Mouse Small Intestine lysates (calculated MW of 38.1kDa according to NP_035518.1). Recommended concentration: 0.5-1.5ug/ml.
Western Blot: Approx 35kDa band observed in Mouse Small Intestine lysates (calculated MW of 38.1kDa according to NP_035518.1). Recommended concentration: 0.5-1.5ug/ml.
Immunogen
Peptide with sequence C-DETNKGFQPDEK, from the C Terminus of the protein sequence according to NP_035518.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.5ug/ml) staining of Mouse Small Intestine lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Mouse Small Intestine lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,134 Da
NCBI Official Full Name
ileal sodium/bile acid cotransporter
NCBI Official Synonym Full Names
solute carrier family 10, member 2
NCBI Official Symbol
Slc10a2
NCBI Official Synonym Symbols
ASBT; ISBT
NCBI Protein Information
ileal sodium/bile acid cotransporter
UniProt Protein Name
Ileal sodium/bile acid cotransporter
Protein Family
UniProt Gene Name
Slc10a2
UniProt Synonym Gene Names
Ntcp2; ASBT; IBAT; ISBT
UniProt Entry Name
NTCP2_MOUSE
Uniprot Description
ASBT: Plays a critical role in the sodium-dependent reabsorption of bile acids from the lumen of the small intestine. Plays a key role in cholesterol metabolism. Defects in SLC10A2 are a cause of primary bile acid malabsorption (PBAM). PBAM is an idiopathic intestinal disorder associated with congenital diarrhea, steatorrhea, interruption of the enterohepatic circulation of bile acids, and reduced plasma cholesterol levels. Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.
Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass
Cellular Component: apical plasma membrane; integral to membrane; integral to plasma membrane; membrane; microvillus; nucleus; proteasome complex
Molecular Function: bile acid:sodium symporter activity; symporter activity
Biological Process: bile acid and bile salt transport; ion transport; sodium ion transport; transport
Research Articles on Slc10a2
Similar Products
Product Notes
The Slc10a2 slc10a2 (Catalog #AAA422212) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Slc10a2 (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Slc10a2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 35kDa band observed in Mouse Small Intestine lysates (calculated MW of 38.1kDa according to NP_035518.1). Recommended concentration: 0.5-1.5ug/ml. Researchers should empirically determine the suitability of the Slc10a2 slc10a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DETNKGFQPD EK. It is sometimes possible for the material contained within the vial of "Slc10a2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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