Rabbit DPP-6 Polyclonal Antibody | anti-DPP-6 antibody

Anti-DPP-6 (DPPX, Dipeptidyl Peptidase-6, Dipeptidyl Aminopeptidase-6, Potassium Channel Accelerating Factor) Catalytic Domain Antibody

Cat. Num. AAA4157545

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: DPP-6; Polyclonal Antibody; Anti-DPP-6 (DPPX; Dipeptidyl Peptidase-6; Dipeptidyl Aminopeptidase-6; Potassium Channel Accelerating Factor) Catalytic Domain Antibody; anti-DPP-6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody will recognize the three longest forms of DPP6 but not the 353 amino acid form. It will not recognize the other family members.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-DPP-6 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Predicted molecular weight: 98 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.
• Immunogen: Synthetic peptide based on the catalytic domain of human DPP6.
• Cellular Localization: Cell Membrane; Single-Pass Type II Membrane Protein
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-DPP-6 antibody

Description: The antibody is a rabbit polyclonal antibody made to the Type-II serine protease DPP-6. The antibody is made to a synthetic peptide based on the catalytic domain of human DPP-6. The antibody reacts with human, mouse and rat DPP-6, and does not recognize other DPP family members. The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant. DPP-6 (DPP-X, also known as potassium channel accelerating factor Dppx) is a Type-II serine proteinase of the clan SC. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa-Gly orientation. DPP-6 has an Asp instead of Ser in the catalytic site. DPP-6 is a member of a broader family of dipeptidyl peptidases including DPP-4, FAP/Seprase, DPP-2, DPP-8, DPP-9, DPP-10, which have differing substrate specificity and tissue localizations. The surface-bound DPP-6 is a homodimer, and cleavage of in the stalk region releases a shed form of DPP-6. The shed is the form found in serum. DPP-6 has been found in highest abundance in the brain, but also in the kidney, liver and lung. A long and short form (DPP-6L, DPP-6S) were initially described, the shorter form with a more compact cytoplasmic domain. Four different human sequences are reported to date; 865, 803, 801 and 353 amino acids in length. The full-length human DPP-6 is 865 amino acids in length, with predicted mass of 97.59 kDa, and pI of 5.86. The 803 and 801 amino acid forms differ only in the cytoplasmic domain, and are predicted at 91.3 and 91.2 kDa respectively, with pIs of 5.88 and 6.05. The shortest form has a cytoplasmic domain identical to the 865 amino acid form, but diverges after the stalk domain, and does not contain the catalytic domain. It is unclear if the short form produces a protein, or the relative distribution of the different forms. RP3-DPP-6 will recognize the three longest forms of DPP-6, but not the 353 amino acid form. In other enzyme systems differential cytoplasmic domains associate with different messenger proteins, and work through different activation pathways. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

Relevance: DPP6 (DPPX, also known as potassium channel accelerating factor Dppx) is a Type-II serine proteinase of the clan SC. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa-Gly orientation. DPP6 has an Asp instead of Ser in the catalytic site. DPP6 is a member of a broader family of dipeptidyl peptidases including DPP4, FAP/Seprase, DPP2, DPP8, DPP9, DPP10, which have differing substrate specificity and tissue localizations. The surface-bound DPP6 is a homodimer, and cleavage of in the stalk region releases a shed form of DPP6. The shed is the form found in serum. DPP6 has been found in highest abundance in the brain, but also in the kidney, liver and lung.