Testing Data
Exonuulease III Enzyme
Exonuclease III, E Coli
Applications
Generation of intermediates for site-directed mutagenesisPreparation of strand-specific radio labelled probes
Preparation of single-stranded DNA
Preparation of single-stranded templates for dideoxy-sequencing of DNA
Creation of unidirectional
Synonyms
Exonuulease III; Exonuclease III; E Coli; Exonuulease III enzyme
Applicable Applications for Exonuulease III enzyme
Generation of intermediates for site-directed mutagenesis
Preparation of strand-specific radio labelled probes
Preparation of single-stranded DNA
Preparation of single-stranded templates for dideoxy-sequencing of DNA
Creation of unidirectional
Preparation of strand-specific radio labelled probes
Preparation of single-stranded DNA
Preparation of single-stranded templates for dideoxy-sequencing of DNA
Creation of unidirectional
Storage Buffer
20 mM Tris-HCl (pH 7.5), 100 mM KCl, 1 mM DTT, and 50% (v/v) Glycerol.
Notes
One unit is defined as the amount of Exonuclease III, E Coli that is required to catalyze the release of 1 nmol of acid soluble nucleotides from double stranded DNA in 30 minutes at 37 degree C in 1X Exonuclease III Reaction Buffer.
Preparation and Storage
Store all components at -20 degree C.
Testing Data
Testing Data
Related Product Information for Exonuulease III enzyme
Exonuclease III, E Coli digests duplex DNA in the 3'†'5' direction from nicked DNA, blunt end DNA, 3'-recessed ends, or 3'-overhangs of less than four bases, and yields nucleoside 5'-phosphates. The DNA degradation proceeds at a uniform rate and produces stretches of ssDNA on the opposite strand. Under defined reaction conditions, the reaction can yield predictable and reproducible digestion results. Conditions such as temperature, ionic strength, template DNA sequence and the Exonuclease III to DNA ratio need to be optimized to suit specific applications to achieve the desired excision rate. Exonuclease III, E Coli is also capable of degrading DNA from 3'-phosphate ends due to intrinsic 3'-phosphatase activity. The enzyme also has apurinic DNA endonuclease activity as well as RNase H activity.
Similar Products
Product Notes
The Exonuulease III (Catalog #AAA4156429) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Exonuulease III can be used in a range of immunoassay formats including, but not limited to, Generation of intermediates for site-directed mutagenesis Preparation of strand-specific radio labelled probes Preparation of single-stranded DNA Preparation of single-stranded templates for dideoxy-sequencing of DNA Creation of unidirectional. Researchers should empirically determine the suitability of the Exonuulease III for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Exonuulease III, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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