Testing Data
RNase III Enzyme | rnc enzyme
RNase III, E Coli
Applications
Gel Super Shift Assay
Synonyms
RNase III; E Coli; rnc enzyme
Sequence Length
242
Applicable Applications for rnc enzyme
dsRNA digestion to short dsRNA fragments
Useful for RNA processing, maturation and structure studies
Directed cleavage of RNA
siRNA preparation for RNA interference in mammalian cells
Note: This product is not suitable for NGS or second strand
Useful for RNA processing, maturation and structure studies
Directed cleavage of RNA
siRNA preparation for RNA interference in mammalian cells
Note: This product is not suitable for NGS or second strand
Storage Buffer
50 mM Tris-HCl (pH 7.5), 0.5M NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100 and 50% (v/v) Glycerol.
Notes
One unit is defined as the amount of RNase III, E Coli that is required to hydrolyze 1 nmol of poly(A) :poly(U) dsRNA in 30 minutes at 37 degree C in 1X RNase III, E Coli Reaction Buffer.
Preparation and Storage
Store at -20 degree C.
Testing Data
Testing Data
Related Product Information for rnc enzyme
RNase III, E Coli is an endoribonuclease that specifically digests dsRNA into dsRNA fragments of 12-15 base pairs that have two or three nucleotide 3'-overhangs with 5' phosphate and 3' hydroxyl termini.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
26,498 Da
NCBI Official Full Name
RNAse III
UniProt Protein Name
Ribonuclease 3
UniProt Gene Name
rnc
UniProt Synonym Gene Names
RNase III
Uniprot Description
Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.
Similar Products
Product Notes
The RNase III rnc (Catalog #AAA4156432) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RNase III can be used in a range of immunoassay formats including, but not limited to, dsRNA digestion to short dsRNA fragments Useful for RNA processing, maturation and structure studies Directed cleavage of RNA siRNA preparation for RNA interference in mammalian cells Note: This product is not suitable for NGS or second strand. Researchers should empirically determine the suitability of the RNase III rnc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNase III, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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