Chromatin Immunoprecipitation (ChIP)
(Histone H2Av antibody (mAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from Drosophila cells and 5 ug of H2Av antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Mouse anti-Drosophila Histone H2Av Monoclonal Antibody | anti-H2Av antibody
Histone H2Av antibody (mAb)(Clone 10E9.D1)
Reactivity
Drosophila
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot
Purity
Protein A Chromatography
Synonyms
Histone H2Av; Monoclonal Antibody; Histone H2Av antibody (mAb)(Clone 10E9.D1); anti-H2Av antibody
Host
Mouse
Reactivity
Drosophila
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
10E9.D1
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H2Av antibody
Chromatin Immunoprecipitation (ChIP), Western Blot (WB)
Application Notes
Applications Validated:
ChIP: 5ug per ChIP
WB: 0.5-2ug/ml dilution
ChIP: 5ug per ChIP
WB: 0.5-2ug/ml dilution
Immunogen
This antibody was raised against a peptide in the C-terminus of the Drosophila melanogaster Histone variant H2Av.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(Histone H2Av antibody (mAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from Drosophila cells and 5 ug of H2Av antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Chromatin Immunoprecipitation (ChIP)
(Histone H2Av antibody (mAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from Drosophila cells and 5 ug of H2Av antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Western Blot (WB)
(Histone H2Av antibody (mAb) tested by Western blot. Detection of Histone H2Av by Western blot. The analysis was performed using Schneider’s Drosophila L2 nuclear extract (20ug)and the Histone H2Av antibody at a dilution of 1ug/ml.)
Western Blot (WB)
(Histone H2Av antibody (mAb) tested by Western blot. Detection of Histone H2Av by Western blot. The analysis was performed using Schneider’s Drosophila L2 nuclear extract (20ug)and the Histone H2Av antibody at a dilution of 1ug/ml.)
Related Product Information for anti-H2Av antibody
Short Description: Our Histone H2Av antibody (mAb)(Clone 10E9.D1) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation and Western blot; it has been shown to react with Drosophila samples.
Background: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle.In Drosophila, the H2A variant corresponding to H2AZ is H2Av. H2Av is an essential protein in Drosophila and has been implicated in both activation and repression of transcription. H2Av is localized to centromeric heterochromatin in Drosophila and flies lacking H2Av have reduced levels of heterochromatin components at the centromeres. However, H2Av nucleosome distribution throughout the rest of the Drosophila genome correlates with genes that have an open and uniform chromatin architecture at promoter regions.
Background: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle.In Drosophila, the H2A variant corresponding to H2AZ is H2Av. H2Av is an essential protein in Drosophila and has been implicated in both activation and repression of transcription. H2Av is localized to centromeric heterochromatin in Drosophila and flies lacking H2Av have reduced levels of heterochromatin components at the centromeres. However, H2Av nucleosome distribution throughout the rest of the Drosophila genome correlates with genes that have an open and uniform chromatin architecture at promoter regions.
Product Categories/Family for anti-H2Av antibody
NCBI and Uniprot Product Information
Molecular Weight
15kDa
Protein Family
Similar Products
Product Notes
The H2Av (Catalog #AAA389035) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Histone H2Av antibody (mAb)(Clone 10E9.D1) reacts with Drosophila and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2Av can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Western Blot (WB). Applications Validated: ChIP: 5ug per ChIP WB: 0.5-2ug/ml dilution. Researchers should empirically determine the suitability of the H2Av for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2Av, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
