Chromatin Immunoprecipitation (ChIP)
(LXR-b pAb tested by ChIP-Chip. ChIP was performed using the ChIP-IT High Sensitivity Kit with chormatin from 2.5 million primary human liver cells. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image is zoomed in to show LXR-b binding at a known LXR binding site in the RARB promoter.)
Rabbit anti-Human LXR-beta Polyclonal Antibody | anti-LXR-beta antibody
LXR-beta antibody (pAb)
ChIP: 10ul per ChIP
ChIP-Seq: 10ul each
WB: 1:500-1:1,000 dilution
Chromatin Immunoprecipitation (ChIP)
(LXR-b pAb tested by ChIP-Chip. ChIP was performed using the ChIP-IT High Sensitivity Kit with chormatin from 2.5 million primary human liver cells. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image is zoomed in to show LXR-b binding at a known LXR binding site in the RARB promoter.)
Chromatin Immunoprecipitation (ChIP)
(LXR-b pAb tested by ChIP-Chip. ChIP was performed using the ChIP-IT High Sensitivity Kit with chormatin from 2.5 million primary human liver cells. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image is zoomed in to show LXR-b binding at a known LXR binding site in the RARB promoter.)
Chromatin Immunoprecipitation (ChIP)
(LXR-b pAb tested by ChIP-qPCR. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 25 ug of human liver chromatin and 10 ul of LXR-b antibody. ChIP DNA was used in qPCR with the negative control primer pairs and primers against the known LXR binding site in the RARB promoter. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Chromatin Immunoprecipitation (ChIP)
(LXR-b pAb tested by ChIP-qPCR. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 25 ug of human liver chromatin and 10 ul of LXR-b antibody. ChIP DNA was used in qPCR with the negative control primer pairs and primers against the known LXR binding site in the RARB promoter. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Western Blot (WB)
(LXR-b pAb tested by Western blot. Detection of LXR-b by Western blot analysis. HeLa whole-cell extract (20 ug) was probed with LXR-b pAb at a 1:1000 dilution.)
Western Blot (WB)
(LXR-b pAb tested by Western blot. Detection of LXR-b by Western blot analysis. HeLa whole-cell extract (20 ug) was probed with LXR-b pAb at a 1:1000 dilution.)
Background: LXR-b (Liver X receptor beta, NR1H2, NER, UNR) is an orphan nuclear receptor and a member of the NR1 receptor family. The LXR receptors (including LXR-a and LXR-b) bind oxysterols and play key roles in maintaining cholesterol homeostasis in macrophages, primarily by regulating multiple components of the reverse cholesterol transport. They also are potent inhibitors of inflammation and are capable of repressing cytokine and chemokine production by Toll-like receptor (TLR)-activated macrophages. LXR-b is more widely expressed than LXR-a, particularly in the brain.
NCBI and Uniprot Product Information
Uniprot Description
LXR-beta: Orphan receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Forms a heterodimer with RXR. Ubiquitous. Belongs to the nuclear hormone receptor family. NR1 subfamily.
Protein type: Nuclear receptor
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: retinoid X receptor binding; ligand-dependent nuclear receptor activity; protein binding; DNA binding; zinc ion binding; steroid hormone receptor activity; apolipoprotein A-I receptor binding; ATPase binding
Biological Process: negative regulation of lipid transport; negative regulation of proteolysis; retinoic acid receptor signaling pathway; positive regulation of fatty acid biosynthetic process; transcription initiation from RNA polymerase II promoter; positive regulation of cellular protein metabolic process; cellular lipid metabolic process; negative regulation of pinocytosis; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cholesterol transport; cholesterol homeostasis; positive regulation of lipoprotein lipase activity; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
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Product Notes
The LXR-beta nr1h2 (Catalog #AAA388794) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LXR-beta antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LXR-beta can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Western Blot (WB). Applications Validated: ChIP: 10ul per ChIP ChIP-Seq: 10ul each WB: 1:500-1:1,000 dilution. Researchers should empirically determine the suitability of the LXR-beta nr1h2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LXR-beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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