Western Blot (WB)
(IKKi/IKKe pAb tested by Western blot. IKKi/IKKe detection by Western blot. The analysis was performed using 10 ug of whole-cell extract from Daudi cells and IKKi/IKKe pAb at a 2 ug/ml dilution.)
Mouse anti-Human IKKi/IKKe Polyclonal Antibody | anti-IKKi/IKKe antibody
IKKi/IKKe antibody (pAb)
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
IKKi/IKKe; Polyclonal Antibody; IKKi/IKKe antibody (pAb); IKBKE; IKK-E; IKK-i; anti-IKKi/IKKe antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Concentration
0.5ug/ul (varies by lot)
Applicable Applications for anti-IKKi/IKKe antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Immunogen
This IKKi/IKKe antibody was raised against a synthetic peptide corresponding to amino acid residues 175-188, 525-540, and 567-580 of human IKKi/IKKe.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(IKKi/IKKe pAb tested by Western blot. IKKi/IKKe detection by Western blot. The analysis was performed using 10 ug of whole-cell extract from Daudi cells and IKKi/IKKe pAb at a 2 ug/ml dilution.)
Western Blot (WB)
(IKKi/IKKe pAb tested by Western blot. IKKi/IKKe detection by Western blot. The analysis was performed using 10 ug of whole-cell extract from Daudi cells and IKKi/IKKe pAb at a 2 ug/ml dilution.)
Related Product Information for anti-IKKi/IKKe antibody
Short Description: Our IKKi/IKKe antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: IKKi/IKKe-IkB Kinase-epsilon (also called IKKi) is a homolog of the IKK (IkB kinase) catalytic subunits IKKa and IKKb. IKKe is primarily expressed in immune cells and may be responsible for modulation of NFkB (NFkB p50 & NFkB p65) activity in the immune system.
Background: IKKi/IKKe-IkB Kinase-epsilon (also called IKKi) is a homolog of the IKK (IkB kinase) catalytic subunits IKKa and IKKb. IKKe is primarily expressed in immune cells and may be responsible for modulation of NFkB (NFkB p50 & NFkB p65) activity in the immune system.
Product Categories/Family for anti-IKKi/IKKe antibody
NCBI and Uniprot Product Information
Molecular Weight
80kDa
Similar Products
Product Notes
The IKKi/IKKe (Catalog #AAA388698) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IKKi/IKKe antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IKKi/IKKe can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1-2ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the IKKi/IKKe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IKKi/IKKe, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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