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Chromatin Immunoprecipitation (ChIP)
(Histone H3 acetyl Lys23 pAb tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of Histone H3 acetyl Lys23 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Rabbit Histone H3K23ac Polyclonal Antibody | anti-H3K23ac antibody
Histone H3K23ac antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Dot Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Synonyms
Histone H3K23ac; Polyclonal Antibody; Histone H3K23ac antibody (pAb); anti-H3K23ac antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H3K23ac antibody
Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
ChIP: 10ul per ChIP
ICC/IF: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
ChIP: 10ul per ChIP
ICC/IF: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
Conjugation
Acetylated
Immunogen
This Histone H3 acetyl Lys23 antibody was raised against a peptide including acetyl-lysine 23 of histone H3.
Abs Residue
Lys
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(Histone H3 acetyl Lys23 pAb tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of Histone H3 acetyl Lys23 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Chromatin Immunoprecipitation (ChIP)
(Histone H3 acetyl Lys23 pAb tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of Histone H3 acetyl Lys23 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Immunofluorescence (IF)
(Histone H3 acetyl Lys23 pAb tested by immunofluorescence. Staining of HeLa cells with Histone H3 acetyl Lys23 pAb (1:500 dilution, top panel) and DAPI (bottom panel).)
Immunofluorescence (IF)
(Histone H3 acetyl Lys23 pAb tested by immunofluorescence. Staining of HeLa cells with Histone H3 acetyl Lys23 pAb (1:500 dilution, top panel) and DAPI (bottom panel).)
Western Blot (WB)
(Histone H3 acetyl Lys23 pAb tested by Western blot. HeLa acid extract probed with Histone H3 acetyl Lys23 polyclonal antibody (1:5,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.)
Western Blot (WB)
(Histone H3 acetyl Lys23 pAb tested by Western blot. HeLa acid extract probed with Histone H3 acetyl Lys23 polyclonal antibody (1:5,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.)
Dot Blot (DB)
(Histone H3K23ac pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3K23ac antibody for acetyl Lys23 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:2,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: H3K4ac peptide. Lane 2: Unmodified H3K4 peptide. Lane 3: H3K9ac peptide. Lane 4: Unmodified H3K9 peptide. Lane 5: H3K18ac peptide. Lane 6: Unmodified H3K18 peptide. Lane 7: H3K23ac peptide. Lane 8: Unmodified H3K23 peptide. Lane 9: H3K27ac peptide. Lane 10: Unmodified H3K27 peptide.)
Dot Blot (DB)
(Histone H3K23ac pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3K23ac antibody for acetyl Lys23 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:2,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: H3K4ac peptide. Lane 2: Unmodified H3K4 peptide. Lane 3: H3K9ac peptide. Lane 4: Unmodified H3K9 peptide. Lane 5: H3K18ac peptide. Lane 6: Unmodified H3K18 peptide. Lane 7: H3K23ac peptide. Lane 8: Unmodified H3K23 peptide. Lane 9: H3K27ac peptide. Lane 10: Unmodified H3K27 peptide.)
Related Product Information for anti-H3K23ac antibody
Short Description: Our Histone H3K23ac antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Dot blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. In estrogen-responsive genes, histone H3 Lys18 is acetylated by CBP/p300 following estrogen stimulation, leading to acetylation of histone H3 Lys23 and methylation of Arg17 by CARM1. These events lead to transcriptional activation of the genes.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. In estrogen-responsive genes, histone H3 Lys18 is acetylated by CBP/p300 following estrogen stimulation, leading to acetylation of histone H3 Lys23 and methylation of Arg17 by CARM1. These events lead to transcriptional activation of the genes.
Product Categories/Family for anti-H3K23ac antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3K23ac (Catalog #AAA388380) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3K23ac antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3K23ac can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB). Applications Validated: ChIP: 10ul per ChIP ICC/IF: 1:500-1:1,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. Researchers should empirically determine the suitability of the H3K23ac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3K23ac, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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