Rabbit Histone H3K27ac Polyclonal Antibody | anti-H3K27ac antibody

Histone H3K27ac antibody (pAb)

Cat. Num. AAA388381

• Reactivity: Budding Yeast, Human, Wide Range Predicted
• Applications: Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation, Dot Blot, Western Blot
• Synonyms: Histone H3K27ac; Polyclonal Antibody; Histone H3K27ac antibody (pAb); anti-H3K27ac antibody

• Host: Rabbit
• Reactivity: Budding Yeast, Human, Wide Range Predicted
• Clonality: Polyclonal
• Form/Format: Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version of this antibody that was purified by Protein A Chromatography is also available.

• Applicable Applications for anti-H3K27ac antibody: Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Cleavage Under Targets and Tagmentation (CUT&Tag), Dot Blot (DB), Western Blot (WB)
• Application Notes: Applications Validated:; ChIP: 3ul per ChIP; ChIP-Seq: 5ul each; WB: 1:1,000-1:5,000 dilution; Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. For Histone H3K27ac, we also offer AbFlex Histone H3K27ac Recombinant Antibody (rAb). For details
• Conjugation: Acetylated
• Immunogen: This Histone H3 acetyl Lys27 antibody was raised against a peptide including acetyl-lysine 27 of histone H3.
• Abs Residue: Lys
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Chromatin Immunoprecipitation (ChIP)

(Histone H3K27ac antibody (pAb) tested by ChIP Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (2.1 x 106 cell equivalents per ChIP) using 3 ul of Histone H3 acetyl Lys27 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using primer pairs specific for the indicated gene as well as a negative control primer pair. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)

Western Blot (WB)

(Histone H3K27ac antibody (pAb) tested by Western blot. HeLa acid extract probed with Histone H3 acetyl Lys27 polyclonal antibody (1:1,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.)

Dot Blot (DB)

(Histone H3K27ac antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3K27ac antibody (pAb) for acetyl Lys27 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: H3K37ac. Lane 2: H3K36ac. Lane 3: H3K9ac. Lane 4: H3K14ac. Lane 5: H3K18ac. Lane 6: H3K23ac. Lane 7: unmod H3K27. Lane 8: H3K27ac. Lane 9: H4K5ac. Lane 10: H4K8ac. Lane 11: H4K12ac. Lane 12: H4K16ac.)

Related Product Information for anti-H3K27ac antibody

Short Description: Our Histone H3K27ac antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, CUT&Tag, Dot blot and Western blot; it has been shown to react with Budding Yeast and Human samples, but it is predicted that it will react with a wide range of sample types.

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H3 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as CBP/p300. Acetylation of histone H3 at Lys27 is associated with transcriptional activation. Histone H3K27 can also be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.

Product Categories/Family for anti-H3K27ac antibody

Primary Antibodies

NCBI and Uniprot Product Information

• Molecular Weight: 17kDa