Rabbit ACSS1 Polyclonal Antibody | anti-ACSS1 antibody

ACSS1 Antibody

Cat. Num. AAA9612542

• Gene Names: ACSS1; ACAS2L; ACECS1; AceCS2L
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: ACSS1; Polyclonal Antibody; ACSS1 Antibody; ACAS2L; AceCS2; AceCS2L; Acetate CoA ligase 2; Acetate--CoA ligase 2; Acetyl CoA synthetase 2; Acetyl Coenzyme A synthetase 2 (AMP forming) like; Acetyl coenzyme A synthetase 2 like; mitochondrial; Acetyl-CoA synthetase 2; Acetyl-coenzyme A synthetase 2-like; ACS2L_HUMAN; Acss1; Acyl CoA synthetase short chain family member 1; Acyl-CoA synthetase short-chain family member 1; FLJ45659; KIAA1846; MGC33843; anti-ACSS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ACSS1 Antibody detects endogenous levels of total ACSS1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ACSS1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human ACSS1 around the acetylation site of Lys642.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Reversibly acetylated on Lys-642. The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylase SIRT3.
• Subunit Structure: Interacts with SIRT3.
• Similarity: Belongs to the ATP-dependent AMP-binding enzyme family.
• Subcellular Location: Mitochondrion matrix.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from TSA treated samples, using Acetyl-ACSS1 (Lys642) Antibody.Lane1: COLO205 cell lysates,Lane2: Hybridoma cell lysates,Lane3: MCF7 cell lysates,Lane4: 293 cell lysates,Lane5: Myeloma cell lysates,Lane6: K652 cell lysates.)

Immunohistochemistry (IHC)

(Staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Related Product Information for anti-ACSS1 antibody

Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity (By similarity). Provides acetyl-CoA that is utilized mainly for oxidation under ketogenic conditions (By similarity). Involved in thermogenesis under ketogenic conditions, using acetate as a vital fuel when carbohydrate availability is insufficient (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 358248205
• NCBI GeneID: 84532
• NCBI Accession #: NP_001239604.1
• NCBI GenBank Nucleotide #: NM_001252675.1
• UniProt Accession #: Q9NUB1; Q5TF42; Q8IV99; Q8N234; Q96JI1; Q96JX6; Q9NU28; B3KXL2; B4DJZ3; D3DW48; F5H6F4; F8W7Y1
• Molecular Weight: 74,857 Da
• NCBI Official Full Name: acetyl-coenzyme A synthetase 2-like, mitochondrial isoform 2
• NCBI Official Synonym Full Names: acyl-CoA synthetase short-chain family member 1
• NCBI Official Symbol: ACSS1
• NCBI Official Synonym Symbols: ACAS2L; ACECS1; AceCS2L
• NCBI Protein Information: acetyl-coenzyme A synthetase 2-like, mitochondrial; acetate--CoA ligase 2
• UniProt Protein Name: Acetyl-coenzyme A synthetase 2-like, mitochondrial
• Protein Family: Acetyl-coenzyme A synthetase
• UniProt Gene Name: ACSS1
• UniProt Synonym Gene Names: ACAS2L; KIAA1846; AceCS2
• UniProt Entry Name: ACS2L_HUMAN

NCBI Description

This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

ACSS1: Important for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions. Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2). Belongs to the ATP-dependent AMP-binding enzyme family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.2.1.1; Carbohydrate Metabolism - pyruvate; Ligase; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: mitochondrial matrix

Molecular Function: protein binding; acetate-CoA ligase activity; ATP binding; AMP binding

Biological Process: acetyl-CoA biosynthetic process from acetate; xenobiotic metabolic process; acetyl-CoA biosynthetic process; propionate biosynthetic process; ethanol oxidation; acetate biosynthetic process