Rabbit Factor VIII-Related Antigen Polyclonal Antibody | anti-FVIII antibody

Factor VIII-Related Antigen

Cat. Num. AAA373010

• Reactivity: Human, Canine, Feline.
• Synonyms: Factor VIII-Related Antigen; Polyclonal Antibody; anti-FVIII antibody

• Host: Rabbit
• Reactivity: Human, Canine, Feline.
• Clonality: Polyclonal
• Form/Format: Factor VIII antibody is a purified immunoglobulin fraction of rabbit antiserum, diluted in phosphate buffered saline, pH 7.6, with protein, and preserved with sodium azide.

• Control: Skin, Placenta
• Localization: Cytoplasmic
• Immunogen: Purified human Factor VII
• Reactivity Note: Paraffin, Frozen
• Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides ; 2. Air dry for 2 hours at 58° C. ; 3. Deparaffinize, dehydrate and rehydrate tissues. ; 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate . ; ; 5. Any of three heating methods may be used: ; ; a. TintoRetriever r Pressure Cooker or Equivalent; Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.; b. TintoRetriever PT Module or Water Bath Method ; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA ; Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.; c. Conventional Steamer Method; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA ; Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. ; ; 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. ; 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. ; 8. Wash slides with IHC wash buffer or DI water. ; 9. Continue IHC staining protocol
• Precautions: 1. For professional users only. Ensure results are interpreted by a medical professional.; 2. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, ?ush with large volumes of water to prevent sodium azide build-up.; 3. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents.; 4. Unused solution should be disposed of according to local and federal regulations.; 5. Do not ingest reagent. If reagent ingested, contact a poison control center immediately.; 6. Avoid contact with eyes. If contact occurs, flush with large quantities of water.; 7. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar). ; 8. For additional safety information refer to Safety Data Sheet for this product.; 9. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL”
• Specimen Preparation: Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. Tissue should remain hydrated via use of Immuno/DNA Washer solutions.
• Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
• Preparation and Storage: Store at 2-8 °C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

Dilution Information

Immunohistochemistry (IHC)

(Insert: IHC of Factor VIII on a FFPE Placenta Tissue)

Testing Data (TD)

Testing Data (TD)

Related Product Information for anti-FVIII antibody

This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.


Factor VIII (F VIII) is an essential clotting factor. The lack of normal F VIII causes Hemophilia A, an inherited bleeding disorder. FVIII is a glycoprotein procofactor synthesized and released into the bloodstream by the liver. In the circulating blood, it is mainly bound to von Willebrand factor (vWF, also known as Factor VIII-related antigen) to form a stable complex. Upon activation by thrombin or Factor Xa, it dis-sociates from the complex to interact with Factor IXa, the coagulation cascade. It is a cofactor to Factor IXa in the activation of Factor X, which, in turn, with its cofactor Factor Va, activates more thrombin. Thrombin cleaves fibrinogen into fibrin which polymerizes and crosslinks (using Factor XIII) into a blood clot. This antibody reacts with endothelial cells in normal, reactive, and neoplastic blood cells. F VIII antibody has helped to establish the endothelial nature of some lesions of disputed histogenesis, e.g., Kaposi`s Sarcoma and Cardiac Myxoma. Not all endothelial cells synthesize (or store) this molecule; therefore, it should not be surprising that not all tumors of endothelial differentiation (benign or malignant) react with this antigen.

NCBI and Uniprot Product Information

• NCBI GI #: 4262857
• NCBI Accession #: AAD15005.1
• NCBI GenBank Nucleotide #: S81579.1
• NCBI Official Full Name: factor VIII
• NCBI Official Symbol: FVIII