Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
WNT11: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: proteinaceous extracellular matrix; extracellular space; cytoplasm; extracellular region
Molecular Function: protein binding; frizzled binding; protein kinase activator activity; receptor binding
Biological Process: cell fate commitment; Wnt receptor signaling pathway; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; multicellular organismal development; Wnt receptor signaling pathway through beta-catenin; palate development; signal transduction; positive regulation of transforming growth factor-beta2 production; protein amino acid phosphorylation; bone mineralization; neuron differentiation; osteoblast differentiation; organ morphogenesis; cell-cell signaling; positive regulation of protein kinase activity; positive regulation of stress fiber formation; artery morphogenesis; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; kidney development; negative regulation of cell migration; positive regulation of cell migration; negative regulation of apoptosis
Research Articles on WNT11
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Product Notes
The Mouse WNT11 wnt11 (Catalog #AAA2890968) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2890968 ELISA Kit recognizes Mouse WNT11. It is sometimes possible for the material contained within the vial of "WNT11/Protein Wnt-11, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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