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Typical Testing Data/Standard Curve (for reference only)

Human alkaline phosphatase, placental (Regan isozyme) ELISA Kit | ALPP elisa kit

Human Placental alkaline pkosphatase, PLAP ELISA Kit

Gene Names
ALPP; ALP; PALP; PLAP; PLAP-1
Reactivity
Human
Synonyms
alkaline phosphatase; placental (Regan isozyme); Human Placental alkaline pkosphatase; PLAP ELISA Kit; ALP; FLJ61142; PALP; PLAP; alkaline phosphomonoesterase; glycerophosphatase; placental alkaline phosphatase; ALPP elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human PLAP. No significant cross-reactivity or interference between human PLAP and analogues was observed.
Sequence Length
535
Samples
Serum, plasma and tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
84 pg/ml-2000 pg/ml
Sensitivity
< 34 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ALPP elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for PLAP. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
References
Placenta-derived exosomes continuously increase in maternal circulation over the first trimester of pregnancy. Suchismita Sarker, Katherin Scholz-Romero, Alejandra Perez, Sebastian E Illanes, Murray D Mitchell, Gregory E Rice and Carlos Salomon (2014)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
250
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57,954 Da
NCBI Official Full Name
alkaline phosphatase, placental type preproprotein
NCBI Official Synonym Full Names
alkaline phosphatase, placental
NCBI Official Symbol
ALPP
NCBI Official Synonym Symbols
ALP; PALP; PLAP; PLAP-1
NCBI Protein Information
alkaline phosphatase, placental type; Regan isozyme; glycerophosphatase; alkaline phosphomonoesterase; placental alkaline phosphatase 1; alkaline phosphatase Regan isozyme
UniProt Protein Name
Alkaline phosphatase, placental type
Protein Family
UniProt Gene Name
ALPP
UniProt Synonym Gene Names
PLAP; PLAP-1
UniProt Entry Name
PPB1_HUMAN

NCBI Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ALPP: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, integral; Cell surface; Motility/polarity/chemotaxis; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - folate biosynthesis; Membrane protein, GPI anchor; EC 3.1.3.1

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: cell surface; plasma membrane; integral to membrane

Molecular Function: alkaline phosphatase activity; zinc ion binding; magnesium ion binding

Biological Process: dephosphorylation

Research Articles on ALPP

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Product Notes

The Human ALPP alpp (Catalog #AAA701995) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA701995 ELISA Kit recognizes Human ALPP. It is sometimes possible for the material contained within the vial of "alkaline phosphatase, placental (Regan isozyme), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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