Typical Testing Data/Standard Curve (for reference only)
Mouse Protein Wnt-10b ELISA Kit | Wnt10b elisa kit
Mouse Protein Wnt-10b ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
WNT10B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Defects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6). SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder. Belongs to the Wnt family.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular region; extracellular space; proteinaceous extracellular matrix
Molecular Function: frizzled binding; receptor binding
Biological Process: cell cycle arrest; cell fate commitment; cell-cell signaling; G2/M transition of mitotic cell cycle; lipid metabolic process; multicellular organismal development; myoblast cell differentiation involved in skeletal muscle regeneration; negative regulation of epithelial cell proliferation; negative regulation of fat cell differentiation; negative regulation of transcription from RNA polymerase II promoter; neuron differentiation; organ morphogenesis; positive regulation of anagen; positive regulation of apoptosis; positive regulation of bone mineralization; positive regulation of cell proliferation; positive regulation of epithelial cell differentiation; positive regulation of ossification; positive regulation of osteoblast differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcriptional preinitiation complex assembly; protein stabilization; regulation of fat cell differentiation; regulation of proteasomal ubiquitin-dependent protein catabolic process; regulation of protein metabolic process; regulation of skeletal muscle development; regulation of transcription from RNA polymerase II promoter; sensory perception of taste; signal transduction; skeletal muscle fiber development; skeletal muscle regeneration; smoothened signaling pathway; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin
Research Articles on Wnt10b
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Product Notes
The Mouse Wnt10b wnt10b (Catalog #AAA2882397) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2882397 ELISA Kit recognizes Mouse Wnt10b. It is sometimes possible for the material contained within the vial of "Protein Wnt-10b, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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