Rabbit Toll Like Receptor 9 (TLR9) ELISA Kit | TLR9 elisa kit

Rabbit Toll Like Receptor 9 (TLR9) ELISA Kit

Cat. Num. AAA2702328

• Reactivity: Rabbit
• Synonyms: Toll Like Receptor 9 (TLR9); Rabbit Toll Like Receptor 9 (TLR9) ELISA Kit; CD289; TLR9 elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 9 (TLR9).; No significant cross-reactivity or interference between Toll Like Receptor 9 (TLR9) and analogues was observed.
• Sequence Length: 1032

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.119ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 9 (TLR9) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 9 (TLR9) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLR9 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 9 (TLR9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor 9 (TLR9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor 9 (TLR9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Toll Like Receptor 9 (TLR9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 157057166
• NCBI GeneID: 81897
• NCBI Accession #: NP_112455.2
• NCBI GenBank Nucleotide #: NM_031178.2
• UniProt Accession #: Q9EQU3; Q4L0K3; Q4L0K4; Q99MF2; Q99MQ8; F8VPN5
• Molecular Weight: 116,412 Da
• NCBI Official Full Name: toll-like receptor 9
• NCBI Official Synonym Full Names: toll-like receptor 9
• NCBI Official Symbol: Tlr9
• NCBI Protein Information: toll-like receptor 9
• UniProt Protein Name: Toll-like receptor 9
• Protein Family: Toll-like receptor
• UniProt Gene Name: Tlr9

Uniprot Description

TLR9: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. Interacts with MYD88 via their respective TIR domains. Interacts (via transmembrane domain) with UNC93B1. Interacts with CD300LH; the interaction may promote full activation of TLR9-triggered innate responses. Interacts with BTK. Interacts with CNPY3 and HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum. Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre- dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. Belongs to the Toll-like receptor family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 9|9 F1

Cellular Component: apical plasma membrane; basolateral plasma membrane; cytoplasm; cytoplasmic vesicle; endoplasmic reticulum; endosome; extracellular space; integral component of membrane; lysosome; membrane; phagocytic vesicle; plasma membrane; proteinaceous extracellular matrix

Molecular Function: interleukin-1 receptor binding; protein binding; siRNA binding; transmembrane receptor activity; unmethylated CpG binding

Biological Process: activation of innate immune response; axonogenesis; cytokine production; defense response to Gram-negative bacterium; defense response to virus; I-kappaB kinase/NF-kappaB signaling; I-kappaB phosphorylation; immune response; immune system process; inflammatory response; innate immune response; maintenance of gastrointestinal epithelium; microglial cell activation; MyD88-dependent toll-like receptor signaling pathway; negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; negative regulation of NF-kappaB transcription factor activity; negative regulation of toll-like receptor signaling pathway; positive regulation of autophagy; positive regulation of B cell activation; positive regulation of B cell proliferation; positive regulation of chemokine production; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of immunoglobulin production; positive regulation of inflammatory response; positive regulation of interferon-alpha biosynthetic process; positive regulation of interferon-beta biosynthetic process; positive regulation of interferon-beta production; positive regulation of interferon-gamma biosynthetic process; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of interleukin-18 production; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of JUN kinase activity; positive regulation of MAPK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of NF-kappaB transcription factor activity; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of toll-like receptor 9 signaling pathway; positive regulation of toll-like receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; regulation of B cell activation; regulation of B cell differentiation; regulation of cytokine secretion; regulation of dendritic cell cytokine production; regulation of inflammatory response; regulation of protein amino acid phosphorylation; regulation of toll-like receptor 9 signaling pathway; response to molecule of bacterial origin; response to virus; signal transduction; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; tumor necrosis factor production