Mouse GATA Binding Protein 3 ELISA Kit | GATA3 elisa kit

Mouse GATA Binding Protein 3 ELISA Kit

Cat. Num. AAA762112

• Gene Names: GATA3; HDR; HDRS
• Reactivity: Mouse
• Synonyms: GATA Binding Protein 3; Mouse GATA Binding Protein 3 ELISA Kit; GATA binding protein 3/GATA-binding factor 3/GATA-binding protein 3/HDRMGC5199/MGC2346/MGC5445/trans-acting T-cell-specific transcription factor GATA-3; GATA3 elisa kit

• Reactivity: Mouse
• Sequence Length: 444

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for GATA3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse GATA3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse GATA3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse GATA3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse GATA3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 13905020
• NCBI GeneID: 2625
• Molecular Weight: 48,045 Da
• NCBI Official Full Name: GATA binding protein 3
• NCBI Official Synonym Full Names: GATA binding protein 3
• NCBI Official Symbol: GATA3
• NCBI Official Synonym Symbols: HDR; HDRS
• NCBI Protein Information: trans-acting T-cell-specific transcription factor GATA-3
• UniProt Protein Name: Trans-acting T-cell-specific transcription factor GATA-3
• Protein Family: GATA-binding factor
• UniProt Gene Name: GATA3
• UniProt Entry Name: GATA3_HUMAN

NCBI Description

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]

Uniprot Description

GATA3: a transcriptional regulator central in Th2 cell differentiation. Inhibits breast cancer growth and pulmonary breast cancer metastasis. Represses INK4C transcription, constrains luminal progenitor cell expansion, and suppresses luminal tumorigenesis in the mammary gland. High GATA3 and low INK4C expression predicts a favorable patient outcome in luminal A type breast tumors. IFN-lambda1 (IL-29) inhibits GATA3 expression and suppresses Th2 responses in human T cells. GATA3 haploinsufficiency leads to HDR (hypoparathyroidism, deafness, and renal dysplasia) syndrome. Two alternatively spliced human isoforms have been described.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: nuclear chromatin; nucleolus; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; DNA binding; protein binding; transcription coactivator activity; transcription factor activity; transcription factor binding

Biological Process: anatomical structure formation; anatomical structure morphogenesis; blood coagulation; cell fate commitment; cell fate determination; defense response; ear development; gut development; heart development; kidney development; male gonad development; mesonephros development; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of fat cell differentiation; negative regulation of inflammatory response; negative regulation of mammary gland epithelial cell proliferation; negative regulation of transcription, DNA-dependent; norepinephrine biosynthetic process; organ morphogenesis; pharyngeal system development; phosphoinositide 3-kinase cascade; positive regulation of interleukin-4 production; positive regulation of protein kinase B signaling cascade; positive regulation of signal transduction; positive regulation of T cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of cytokine biosynthetic process; response to estrogen stimulus; response to virus; signal transduction; sympathetic nervous system development; T cell receptor signaling pathway; TOR signaling pathway; transcription from RNA polymerase II promoter; uterus development

Disease: Hypoparathyroidism, Sensorineural Deafness, And Renal Disease