Human Glutamate Receptor, Ionotropic, Delta 2 (GRId2) ELISA Kit | GRId2 elisa kit

Human Glutamate Receptor, Ionotropic, Delta 2 (GRId2) ELISA Kit

Cat. Num. AAA2706847

• Gene Names: GRID2; GluD2; SCAR18
• Reactivity: Human
• Synonyms: Glutamate Receptor; Ionotropic; Delta 2 (GRId2); Human Glutamate Receptor; Delta 2 (GRId2) ELISA Kit; GluD2; GLURD2; GRId2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamate Receptor, Ionotropic, Delta 2 (GRId2).; No significant cross-reactivity or interference between Glutamate Receptor, Ionotropic, Delta 2 (GRId2) and analogues was observed.
• Sequence Length: 912

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.78-50ng/mL
• Sensitivity: < 0.29ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, Delta 2 (GRId2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, Delta 2 (GRId2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRId2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Receptor, Ionotropic, Delta 2 (GRId2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Receptor, Ionotropic, Delta 2 (GRId2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Receptor, Ionotropic, Delta 2 (GRId2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Receptor, Ionotropic, Delta 2 (GRId2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 558472748
• NCBI GeneID: 2895
• NCBI Accession #: NP_001273767.1
• NCBI GenBank Nucleotide #: NM_001286838.1
• UniProt Accession #: O43424
• NCBI Official Full Name: glutamate receptor ionotropic, delta-2 isoform 2
• NCBI Official Synonym Full Names: glutamate ionotropic receptor delta type subunit 2
• NCBI Official Symbol: GRID2
• NCBI Official Synonym Symbols: GluD2; SCAR18
• NCBI Protein Information: glutamate receptor ionotropic, delta-2
• UniProt Protein Name: Glutamate receptor ionotropic, delta-2
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRID2
• UniProt Synonym Gene Names: GLURD2; GluD2; GluR delta-2 subunit

NCBI Description

The protein encoded by this gene is a member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. The encoded protein is a multi-pass membrane protein that is expressed selectively in cerebellar Purkinje cells. A point mutation in the mouse ortholog, associated with the phenotype named 'lurcher', in the heterozygous state leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. This protein also plays a role in synapse organization between parallel fibers and Purkinje cells. Alternate splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause cerebellar ataxia in humans. [provided by RefSeq, Apr 2014]

Uniprot Description

GluR-delta2: Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRID2 subfamily.

Protein type: Channel, calcium; Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4q22.1-q22.2

Cellular Component: cell junction; dendritic spine; integral component of plasma membrane; ionotropic glutamate receptor complex; plasma membrane; postsynaptic membrane; synapse

Molecular Function: extracellular-glutamate-gated ion channel activity; glutamate receptor activity; ionotropic glutamate receptor activity; PDZ domain binding; protein binding

Biological Process: cerebellar granule cell differentiation; excitatory postsynaptic potential; glutamate receptor signaling pathway; heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; ionotropic glutamate receptor signaling pathway; positive regulation of synaptogenesis; prepulse inhibition; regulation of neuron apoptosis; synaptic transmission, glutamatergic; transport

Disease: Spinocerebellar Ataxia, Autosomal Recessive 18