Mouse Toll Like Receptor 9 (TLR9) ELISA Kit | TLR9 elisa kit

Mouse Toll Like Receptor 9 (TLR9) ELISA Kit

Cat. Num. AAA2025543

• Reactivity: Mouse
• Synonyms: Toll Like Receptor 9 (TLR9); Mouse Toll Like Receptor 9 (TLR9) ELISA Kit; CD289; TLR9 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of TLR9. No significant cross-reactivity or interference between TLR9 and analogues was observed.
• Sequence Length: 1032

• Samples: Mouse tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.056ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TLR9 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TLR9 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLR9 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TLR9 in mouse tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TLR9. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TLR9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TLR9, biotin-conjugated antibody and enzymeconjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TLR9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TLR9 elisa kit

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 157057166
• NCBI GeneID: 81897
• NCBI Accession #: NP_112455.2
• NCBI GenBank Nucleotide #: NM_031178.2
• Molecular Weight: 116,412 Da
• NCBI Official Full Name: toll-like receptor 9
• NCBI Official Synonym Full Names: toll-like receptor 9
• NCBI Official Symbol: Tlr9
• NCBI Protein Information: toll-like receptor 9
• UniProt Protein Name: Toll-like receptor 9
• Protein Family: Toll-like receptor
• UniProt Gene Name: Tlr9
• UniProt Entry Name: TLR9_MOUSE

Uniprot Description

TLR9: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. Interacts with MYD88 via their respective TIR domains. Interacts (via transmembrane domain) with UNC93B1. Interacts with CD300LH; the interaction may promote full activation of TLR9-triggered innate responses. Interacts with BTK. Interacts with CNPY3 and HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum. Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre- dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. Belongs to the Toll-like receptor family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: membrane; lysosome; basolateral plasma membrane; endoplasmic reticulum; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; cytoplasmic vesicle; endosome

Molecular Function: protein binding; siRNA binding; interleukin-1 receptor binding; transmembrane receptor activity; unmethylated CpG binding

Biological Process: positive regulation of JNK activity; regulation of cytokine secretion; positive regulation of interleukin-12 production; maintenance of gastrointestinal epithelium; microglial cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of interferon-alpha biosynthetic process; regulation of B cell activation; defense response to Gram-negative bacterium; positive regulation of interleukin-18 production; signal transduction; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to molecule of bacterial origin; negative regulation of interleukin-6 production; positive regulation of interferon-beta production; negative regulation of interleukin-8 production; inflammatory response; defense response to virus; positive regulation of autophagy; regulation of protein amino acid phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; response to virus; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of toll-like receptor signaling pathway; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; regulation of inflammatory response; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; negative regulation of toll-like receptor signaling pathway; innate immune response; immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of inflammatory response; positive regulation of interferon-gamma biosynthetic process