Human TLR9 (Toll-Like Receptor 9) CLIA Kit | TLR9/CD289 clia kit

Human TLR9 (Toll-Like Receptor 9) CLIA Kit

Cat. Num. AAA2531091

• Reactivity: Human
• Synonyms: TLR9 (Toll-Like Receptor 9); Human TLR9 (Toll-Like Receptor 9) CLIA Kit; TLR9/CD289 clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant TLR9/CD289. No significant cross-reactivity or interference between TLR9/CD289 and analogues was observed.
• Sequence Length: 1032

• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLR9/CD289 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of TLR9/CD289 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to TLR9/CD289. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TLR9/CD289 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TLR9/CD289, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of TLR9/CD289. You can calculate the concentration of TLR9/CD289 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 157057166
• NCBI GeneID: 81897
• NCBI Accession #: NP_112455.2
• NCBI GenBank Nucleotide #: NM_031178.2
• Molecular Weight: 116,412 Da
• NCBI Official Full Name: toll-like receptor 9
• NCBI Official Synonym Full Names: toll-like receptor 9
• NCBI Official Symbol: Tlr9
• NCBI Protein Information: toll-like receptor 9
• UniProt Protein Name: Toll-like receptor 9
• UniProt Gene Name: Tlr9
• UniProt Entry Name: TLR9_MOUSE

Uniprot Description

TLR9: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. Interacts with MYD88 via their respective TIR domains. Interacts (via transmembrane domain) with UNC93B1. Interacts with CD300LH; the interaction may promote full activation of TLR9-triggered innate responses. Interacts with BTK. Interacts with CNPY3 and HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum. Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre- dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. Belongs to the Toll-like receptor family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: membrane; lysosome; basolateral plasma membrane; endoplasmic reticulum; apical plasma membrane; cytoplasm; integral to membrane; plasma membrane; cytoplasmic vesicle; endosome

Molecular Function: protein binding; siRNA binding; interleukin-1 receptor binding; transmembrane receptor activity; unmethylated CpG binding

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of JNK activity; regulation of cytokine secretion; regulation of dendritic cell cytokine production; positive regulation of interleukin-12 production; maintenance of gastrointestinal epithelium; microglial cell activation; regulation of B cell activation; positive regulation of interferon-alpha biosynthetic process; positive regulation of NF-kappaB import into nucleus; defense response to Gram-negative bacterium; positive regulation of interleukin-18 production; signal transduction; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to molecule of bacterial origin; negative regulation of interleukin-6 production; positive regulation of interferon-beta production; negative regulation of interleukin-8 production; inflammatory response; defense response to virus; positive regulation of autophagy; tumor necrosis factor production; regulation of protein amino acid phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; response to virus; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of toll-like receptor signaling pathway; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; regulation of inflammatory response; positive regulation of interferon-beta biosynthetic process; negative regulation of toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; immune response; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; positive regulation of inflammatory response; positive regulation of interferon-gamma biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process