Typical Testing Data/Standard Curve (for reference only)
Ferrous Ion Assay Kit
Ferrous Ion Colorimetric Assay Kit
Synonyms
Ferrous Ion; Ferrous Ion Colorimetric Assay Kit; Ferrous Ion assay kit
Samples
Serum, plasma and tissue samples
Detection Range
0.08-50 mg/L
Sensitivity
0.08 mg/L
Preparation and Storage
Store at 2-8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Testing Data
Testing Data
Related Product Information for Ferrous Ion assay kit
Intended Uses: This kit can be used to measure the concentration of ferrous iron in serum, plasma and tissue samples.
Principle of the Assay: Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.
Background/Introduction: Serum iron is an essential element in human body. Iron with physiological activity mainly exists in the form of ferroheme and transferrin in plasma. 65% of the iron in the body is bound up in hemoglobin molecules in red blood cells. About 4% is bound up in myoglobin molecules. Around 30% of the iron in the body is stored as ferritin or hemosiderin in the spleen, the bone marrow and the liver. Small amounts of iron can be found in other molecules in cells throughout the body. None of this iron is directly accessible by testing the serum. However, some iron is circulating in the serum. Transferrin is a molecule produced by the liver that binds one or two iron (III) ions, i.e. ferric iron, Fe3+. Transferrin is essential if stored iron is to be moved and used.
Principle of the Assay: Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.
Background/Introduction: Serum iron is an essential element in human body. Iron with physiological activity mainly exists in the form of ferroheme and transferrin in plasma. 65% of the iron in the body is bound up in hemoglobin molecules in red blood cells. About 4% is bound up in myoglobin molecules. Around 30% of the iron in the body is stored as ferritin or hemosiderin in the spleen, the bone marrow and the liver. Small amounts of iron can be found in other molecules in cells throughout the body. None of this iron is directly accessible by testing the serum. However, some iron is circulating in the serum. Transferrin is a molecule produced by the liver that binds one or two iron (III) ions, i.e. ferric iron, Fe3+. Transferrin is essential if stored iron is to be moved and used.
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Product Notes
The Ferrous Ion (Catalog #AAA2567693) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Ferrous Ion, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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