5'- Phosphoadenylyl- (3' -->5')- guanosine (pApG / 5'-p(rA)p(rG)) Biochemical

5'- Phosphoadenylyl- (3' -->5')- guanosine (pApG/ 5'-p(rA)p(rG)), sodium salt

Cat. Num. AAA256663

• Purity: Typical analysis is >95% (HPLC/ UV/ 256 nm). The product is not sterile and has not been tested for endotoxins
• Synonyms: 5'- Phosphoadenylyl- (3' -->5')- guanosine (pApG / 5'-p(rA)p(rG)); 5'- Phosphoadenylyl- (3' -->5')- guanosine (pApG/ 5'-p(rA)p(rG)); sodium salt; 5'- Phosphoadenylyl- (3' --->5')- guanosine; 5'- Phosphoadenylyl- (3' -->5')- guanosine (pApG / 5'-p(rA)p(rG)) biochemical

• Purity/Purification: Typical analysis is >95% (HPLC/ UV/ 256 nm). The product is not sterile and has not been tested for endotoxins
• Form/Format: Sodium. Crystallized or lyophilized sodium salt.

• Solubility: pApG is soluble to at least 4.8 mM in water, limits have not been determined.
• SMILES: O=C(NC(N)=N1)C2=C1N([C@H]3[C@H](O)[C@H](O)[C@@H](COP(O[C@H]4[C@@H](O)[C@H](N5C=NC6=C5N=CN=C6N)O[C@@H]4COP([O-])([O-])=O)([O-])=O)O3)C=N2.[Na+]
• CAS Number: 5064-50-6
• Formula: C??H??N??O??P?
• Properties: pApG is a linear dinucleotide which was found to be a degradation product of the bacterial second messenger3'3'-cGAMP (c-(ApGp)), generated by specific PDEs in Vibrio cholerae (Gao et al. 2015). pApG does not bind to thec-diGMP-I riboswitch (Smith et al. 2012).
• UV: lambda max 256 nm, epsilon 25050, pH 7
• Notes: Please keep in mind that equal concentrations of the compound may look different in volume due to sensitivity of the lyophilized form to humidity. The compound can even contract to small volume droplets. Normally the product is located in the conical bottom of the tube. Micromolar quantities are determined by UV at lambda max.; Please rinse tube walls carefully and preferably use ultrasonic or vortex to achieve total and uniform mixing. When opening the tube please make sure that no substance is lost within the cap.; Please keep in mind that the in vivo properties of this compound are not sufficiently characterized up to now.
• Preparation and Storage: Store at -20 degree C; It is chemically rather stable. Nevertheless, we recommend that the compound should be stored in the freezer, for longer storage periods preferably in freeze-dried form.

Structure

Related Product Information for 5'- Phosphoadenylyl- (3' -->5')- guanosine (pApG / 5'-p(rA)p(rG)) biochemical

In pApG a 5'-AMP unit is connected with a 5'-GMP unit via a 3' --->5' linkage to form a linear dinucleotide.

References

Gao, J.; Tao, J.; Liang, W.; Zhao, M.; Du, X.; Cui, S.; Duan, H.; Kan, B.;Su, X.; Jiang, Z., Cell Res., 25, 539 - 550 (2015):"Identification and Characterization of Phosphodiesterases that Specifically Degrade 3'3'-cyclic GMP-AMP"
Smith, K.D.; Lipchock, S.V.; Strobel, S.A., Biochemistry, 51, 425 - 432 (2012): "Structural and Biochemical Characterization ofLinear Dinucleotide Analogs Bound to the c-di-GMP-I Aptamer.