P1- (5'- [1, N6- Ethenoadenosyl])- P4- (5'- [1, N6- ethenoadenosyl])- tetraphosphate (epsilon-Ap?-epsilon-A / epsilon-Apppp-epsilon-A) Biochemical

P1- (5'- [1, N6- Ethenoadenosyl])- P4- (5'- [1, N6- ethenoadenosyl])- tetraphosphate (epsilon-Ap?-epsilon-A/ epsilon-Apppp-epsilon-A), sodium salt

Cat. Num. AAA256653

• Purity: Typical analysis is >95% (HPLC/ UV/ 275 nm). The product is not sterile and has not been tested for endotoxins.
• Synonyms: P1- (5'- [1; N6- Ethenoadenosyl])- P4- (5'- [1; N6- ethenoadenosyl])- tetraphosphate (epsilon-Ap?-epsilon-A / epsilon-Apppp-epsilon-A); N6- ethenoadenosyl])- tetraphosphate (epsilon-Ap?-epsilon-A/ epsilon-Apppp-epsilon-A); sodium salt; N6- ethenoadenosyl])- tetraphosphate; N6- ethenoadenosyl])- tetraphosphate (epsilon-Ap?-epsilon-A / epsilon-Apppp-epsilon-A) biochemical

• Purity/Purification: Typical analysis is >95% (HPLC/ UV/ 275 nm). The product is not sterile and has not been tested for endotoxins.
• Form/Format: Tetrasodium. Lyophilized or crystallized sodium salt.

• Solubility: epsilon-Ap?-epsilon-A has good solubility in water and aqueous buffers (>= 10 mM, limits have not been determined).
• SMILES: O[C@H]1[C@@H](O)[C@H](N2C=NC3=C2N=CN4C3=NC=C4)O[C@@H]1COP(OP(OP(OP(OC[C@H]5O[C@@H](N6C=NC7=C6N=CN8C7=NC=C8)[C@H](O)[C@@H]5O)([O-])=O)([O-])=O)([O-])=O)([O-])=O.[Na+].[Na+].[Na+].[Na+]
• CAS Number: 96920-51-3
• Formula: C??H??N??O??P?
• Properties: The etheno groups of epsilon-Ap?-epsilon-A fluoresce with exc 307 nm and em 410 nm.
• UV: lambda max 275 nm, epsilon 10800, pH 7
• Notes: Equal concentrations of epsilon-Ap?-epsilon-A can appear very different in volume due to sensitivity of the lyophilized form to humidity. The compound can even contract to small volume droplets. Normally the product is located in the conical bottom of the tube. Micromolar quantities are determined by UV at lambda max.; Please rinse tube walls carefully and preferably use ultrasonic or vortex to achieve total and uniform mixing.; Please keep in mind that the in vivo properties of this compound are not sufficiently characterized up to now.
• Preparation and Storage: Store at -20 degree C; Protect from light.; It has sufficient stability at room temperature and does not need special care during handling orshipment. Nevertheless, we recommend that the compound should be protected from light, stored in the freezer, for longer storage periods preferably in freeze-dried form.

Structure

Related Product Information for P1- (5'- [1, N6- Ethenoadenosyl])- P4- (5'- [1, N6- ethenoadenosyl])- tetraphosphate (epsilon-Ap?-epsilon-A / epsilon-Apppp-epsilon-A) biochemical

Fluorescent analogue of Ap4A. epsilon-Ap?-epsilon-A is a fluorescent modification of the naturally occurring dinucleoside polyphosphate Ap4A, where twoadenosine moieties are linked via their 5' positions by four phosphate groups. Both adenine nucleobases are modified by fluorescentetheno groups.

References

Rotilan, P.; Ramos, A.; Pintor, J.; Tortes, M.; Mires-Portugal, M.T., FEBS Lett., 280, 371 - 374 (1991): "Di(1,N6-ethenoadenosine)5',5"'-P1,P4-tetraphosphate, a fluorescent enzymatically active derivative of Ap4A"
Gasmi, L.; Cartwright, J.L.; McLennan, A.G., Biochim. Biophys. Acta, 1405, 121 - 127 (1998): "The hydrolytic activity of bovineadrenal medullary plasma membranes towards diadenosine polyphosphates is due to alkaline phosphodiesterase-I".