Rabbit CDC25A Polyclonal Antibody | anti-CDC25A antibody

CDC25A Polyclonal Antibody

Cat. Num. AAA126373

• Gene Names: CDC25A; CDC25A2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: CDC25A; Polyclonal Antibody; CDC25A Polyclonal Antibody; CDC25A2; cdc25A; anti-CDC25A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 524

• Applicable Applications for anti-CDC25A antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human CDC25A
• Calculated Molecular Weight: 59kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat liver using CDC25A antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using CDC25A antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat heart using CDC25A antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver injury using CDC25A antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse kidney using CDC25A antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-CDC25A antibody

The cdc25 protein phosphatase family plays a critical role in activating cyclin-dependent kinases (CDKs) via dephosphorylation of conserved Thr14/Tyr15 inhibitory phosphorylation sites. While cdc25C is primarily responsible for activating CDK1 to overcome the G2/M checkpoint and allow mitotic entry, the primary substrate of cdc25A is CDK2, which, when active, allows progression through the G1/S and intra-S checkpoints (1). Abundance, subcellular localization and activity of cdc25A is tightly controlled by a variety of mechanisms, including phosphorylation, ubiquitination, and inhibitory binding to 14-3-3 proteins. During normal cell cycle progression, elevated c-Myc and E2F transcription factor levels lead to increased cdc25A expression (2). When conditions are favorable for DNA synthesis, cdc25A and CDK2 form an activation loop, wherein each activates the other enzyme (1). DNA damage, on the other hand, leads to multisite phosphorylation at inhibitory sites (Ser123, Ser177, Ser278, Ser292, and Thr506) by Chk1 and Chk2, which result in 14-3-3 binding and ubiquitin-mediated degradation (3,4).

Product Categories/Family for anti-CDC25A antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 50403734
• NCBI GeneID: 993
• UniProt Accession #: P30304; Q8IZH5; Q96IL3; Q9H2F2
• Molecular Weight: 524
• NCBI Official Full Name: M-phase inducer phosphatase 1
• NCBI Official Synonym Full Names: cell division cycle 25A
• NCBI Official Symbol: CDC25A
• NCBI Official Synonym Symbols: CDC25A2
• NCBI Protein Information: M-phase inducer phosphatase 1; CDC25A2-CAG isoform; CDC25 isoform A1-CAG; dual specificity phosphatase CDC25A
• UniProt Protein Name: M-phase inducer phosphatase 1
• Protein Family: M-phase inducer phosphatase
• UniProt Gene Name: CDC25A
• UniProt Entry Name: MPIP1_HUMAN

NCBI Description

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC25A: a member of the MPI phosphatase family. Functions as a dosage-dependent inducer in mitotic control. Required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two splice variant isoforms have been described.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: cell proliferation; mitosis; response to radiation; regulation of cell cycle; cell division; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle